Changes in the electrophoretic mobility of thymus, spleen and lymph node cells in tumor-bearing mice during dynamic tumor growth

• Published in: T͡S︡itologii͡a Vol. 31; no. 2; p. 209
• Main Authors: Grinevich, Iu A, Fil'chakov, F V, Alekseev, S B, Kuz'menko, A P
• Format: Journal Article
• Language: Russian
• Published: Russia (Federation) 01-02-1989
• Subjects: Animals; Carcinoma - immunology; Cell Movement; Electrophoresis; Lung Neoplasms - immunology; Lymph Nodes - cytology; Lymph Nodes - immunology; Lymphocytes - cytology; Lymphocytes - immunology; Male; Melanoma, Experimental - immunology; Mice; Mice, Inbred C57BL; Neoplasm Transplantation; Spleen - cytology; Spleen - immunology; Surface Properties; Thymus Gland - cytology; Thymus Gland - immunology; Time Factors
• ISSN: 0041-3771

Using the analytic microscope "Parmoquant-2" (GDR), histograms were obtained demonstrating electrophoretic mobility (EPM) of lymphoid cells of C57BL/6 mice in the course of growth of the Lewis carcinoma (3LL) and melanoma B16 administered under the skin of the femur. Changes in the average values of EPM of thymic, splenic and lymph nodal cells in the process of tumor growth appeared similar. It is shown that the medium thymocyte EMP is growing towards the terminal stage of tumor growth, at the expense of the decrease in the share of PNA+ cells. Splenic cell bimodal distribution according to EPM became, in the course of tumor growth in intact mice, unimodal with some insignificant decrease in the median EPM values. The median EPM of regional and distant lymph nodes in the process of tumor growth is of phase character. It is supposed that investigation of lymph node EPM could be used for studying tumor growth kinetics.