Use of fluorescein isothiocyanate to label plasma membrane of lymphocytes

The possibility of using fluoresceine isothiocyanate to label plasmatic membranes of lymphocytes was explored. Membrane labeling can be done under physiological conditions. The label interacted with the surface located membrane component, and the binding centers became saturated with the label conce...

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Bibliographic Details
Published in:	Prikladnaja biohimija i mikrobiologija Vol. 15; no. 4; p. 576
Main Authors:	Agibalov, Iu V, Sitkovskiĭ, M V, Baranova, F S, Tsypin, A B, Koelov, Iu P
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-07-1979
Subjects:	Cell Membrane - ultrastructure Fluoresceins Kinetics Lymphocyte Activation Lymphocytes - ultrastructure Mitogens Thiocyanates
Online Access:	Get more information
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Summary:	The possibility of using fluoresceine isothiocyanate to label plasmatic membranes of lymphocytes was explored. Membrane labeling can be done under physiological conditions. The label interacted with the surface located membrane component, and the binding centers became saturated with the label concentration which left the membrane undamaged. Modification of the membrane surface resulting from fluoresceine isothiocyanate treatment influenced significantly intercellular interactions mediating mitogen-induced blast transformation.
ISSN:	0555-1099