Conformational changes in the heat-denatured nucleotide complex of human lymphocytes on subsequent cooling

The microfluorometric technique allowed an assay of the chromatin state from binding of acridine orange in studies on the kinetics of conformational changes in the nucleoprotein complex (NP) of human peripheral blood lymphocytes. Extraction of F1 histone fraction with 0.01 N HCl (pH 2) substantially...

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Bibliographic Details
Published in:	Biulleten' eksperimental'noi biologii i meditsiny Vol. 88; no. 12; p. 680
Main Authors:	Stepanian, A R, Lideman, R R, Zlobina, G P
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-12-1979
Subjects:	Cell Nucleus - metabolism Cells, Cultured Histones - metabolism Humans Kinetics Lymphocytes - metabolism Nucleotides - blood Protein Conformation Protein Denaturation Temperature Viscosity
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Summary:	The microfluorometric technique allowed an assay of the chromatin state from binding of acridine orange in studies on the kinetics of conformational changes in the nucleoprotein complex (NP) of human peripheral blood lymphocytes. Extraction of F1 histone fraction with 0.01 N HCl (pH 2) substantially altered (decelerated) conformational changes in the NP complex. Extraction of F1, F2 and F3 histone fractions with 0.25N HCl (pH 0.6) led to a more pronounced deceleration of the kinetics of conformational changes. The latter ones were decelerated as well when the process was run in a more viscous medium (e. g. in a 70% glycerin solution). No conformational changes in the NP complex deproteinized with 0.25N HCl were revealed in a vicsous medium. In such a case the NP complex showed low F530 values and elevated values of the alpha coefficient that seems likely to suggest the nonreversible denaturation of the NP complex.
ISSN:	0365-9615