Topological model of bacteriophage-T4 DNA replication

Topological model of bacteriophage-T4 DNA replication

The structure and function of the DNA--membrane complex in E. coli cells infected with bacteriophage T4 was studied. The DNA--membrane complex was isolated from the cells pulse or uniformly labeled with 3H-thymidine, fractionated by detergent treatment and separated on a discontinuous sucrose gradie...

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Bibliographic Details
Published in:Molekuliarnaia biologiia Vol. 11; no. 4; p. 766
Main Authors: Boĭkov, P Ia, Shevchenko, N A
Format: Journal Article
Language:Russian
Published: Russia (Federation) 01-07-1977
Subjects:
Cell Membrane - physiology
Coliphages - physiology
DNA Replication
Escherichia coli - physiology
Models, Biological
Polyethylene Glycols
Virus Replication
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Summary:The structure and function of the DNA--membrane complex in E. coli cells infected with bacteriophage T4 was studied. The DNA--membrane complex was isolated from the cells pulse or uniformly labeled with 3H-thymidine, fractionated by detergent treatment and separated on a discontinuous sucrose gradient. The attachment of small DNA fragments to the plasma membrane was analysed. Replicating bacteriophage T4 DNA was reversibly associated with the cytoplasmic membrane in the wall/membrane adhesion zones of the envelope. In the adhesion zone the cell wall, cytoplasmic membrane, DNA, different replicative proteins, probably form the initiation complex resistant to sonication, DNAase and Triton-X-100. This association is probably reversible. The topological aspects of bacteriophage T4 DNA replication are discussed.
ISSN:0026-8984