Specific hypothalamic proteins

• Published in: Voprosy biokhimii mozga Vol. 13; p. 67
• Main Authors: Srapionian, R M, Saakian, S A, Saakian, F M, Galoian, A A
• Format: Journal Article
• Language: Russian
• Published: Armenia (Republic) 1978
• Subjects: Amino Acids - analysis; Animals; Carrier Proteins - isolation & purification; Cattle; Chemical Phenomena; Chemistry; Coronary Vessels - drug effects; Electrophoresis, Disc; Hypothalamus - analysis; Isoelectric Point; Molecular Weight; Nerve Tissue Proteins - isolation & purification; Nerve Tissue Proteins - pharmacology; Vasodilator Agents - isolation & purification
• ISSN: 0507-2972

We had previously shown the presence of four protein fractions in hypothalamus of cattle having a coronarodilatatory effect. In this study we have developed methods for the isolation of these proteins and have studied some of their physicochemical properties, amino acid composition, molecular weight, iso-electric point etc. The results obtained have shown that the methods used enable the isolation of quite pure coronaroactive proteins, the content of which in the hypothalamus is equal to 12 mg/kg of fresh tissue. A study of the amino acid composition of these proteins has shown that they are not of the same type. They also differ in molecular weight, in their electrophoretic properties on 7.5% polyacrylamide gel and their iso-electric points. The possibility of a dual function of these proteins as carriers and precursors of corresponding neurohormones is discussed.