Partial suppression of the effect of gene re1A in Fusr-mutant Escherichia coli K-12 cells

Mutants of Escherichia coli K-12 re1A+-strain CP 78 resistant to fusidic acid (Fusr) were isolated and forms sensitive to high concentration of leucine (500 g/ml) were selected. When shifted down from nutrient broth to minimal medium M9 with supplemented glucose and required amino acids, these leuci...

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Bibliographic Details
Published in:	Genetika Vol. 14; no. 5; p. 890
Main Authors:	Shakulov, R S, Livshits, V A, Zaigraeva, G G, Lideman, L F, Mukhamed'ianova, T D
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-05-1978
Subjects:	Adaptation, Physiological Bacterial Proteins - biosynthesis Drug Resistance, Microbial Escherichia coli - drug effects Escherichia coli - genetics Fusidic Acid - pharmacology Genes Leucine - pharmacology Mutation RNA, Bacterial - biosynthesis
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Summary:	Mutants of Escherichia coli K-12 re1A+-strain CP 78 resistant to fusidic acid (Fusr) were isolated and forms sensitive to high concentration of leucine (500 g/ml) were selected. When shifted down from nutrient broth to minimal medium M9 with supplemented glucose and required amino acids, these leucine-sensitive mutants continued RNA synthesis and demonstrated the prolonged lag-phase in contrast to the parent strain CP 78. Both properties are known to be characteristic of the Rel- strains. At the same time withdrawal of the required amino acids results in cessation of RNA synthesis in Fusr mutants, in the parent Rel+ strain. Thus, leucine-sensitive Fusr mutants show Rel- phenotype only upon amino acid starvation caused by shift down from nutrient broth to minimal medium.
ISSN:	0016-6758