Purification of beta-glucanase on cellulose

The paper describes a method for purifying beta-glucanse (lychenase) which includes enzyme absorption on the microcrystalline cellulose column or ground filter paper at pH 5.5, and elution with phosphate buffer pH 7.6. Specific activity of beta-glucanase eluted from the column may be increased by 8-...

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Bibliographic Details
Published in:	Prikladnaja biohimija i mikrobiologija Vol. 15; no. 6; p. 874
Main Authors:	Tiunova, N A, Zaikina, I V, Fipantene, R K, Avizhenic, V Iu
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-11-1979
Subjects:	Bacillus subtilis - enzymology beta-Glucosidase - isolation & purification beta-Glucosidase - metabolism Cellulose Glucosidases - isolation & purification Kinetics Lichens - enzymology Plants - enzymology
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Summary:	The paper describes a method for purifying beta-glucanse (lychenase) which includes enzyme absorption on the microcrystalline cellulose column or ground filter paper at pH 5.5, and elution with phosphate buffer pH 7.6. Specific activity of beta-glucanase eluted from the column may be increased by 8--23 times, depending on the purification conditions.
ISSN:	0555-1099