Study of the chemical receptor for diphtheria toxin in cell membranes

Adsorption of diphtheria toxin on immobilized cell membrane preparations in the presence of gangliosides was studied. A mixture of gangliosides completely inhibited the adsorption of diphtheria toxin on the cell membranes, both sensitive (HeLa cells, macrophages) and resistant (L-cells) to its actio...

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Bibliographic Details
Published in:	Biulleten' eksperimental'noi biologii i meditsiny Vol. 85; no. 5; p. 549
Main Authors:	Vertiev, Iu V, Ezepchuk, Iu V
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-05-1978
Subjects:	Adsorption Animals Cell Membrane - drug effects Diphtheria Toxin - pharmacology Dose-Response Relationship, Drug Drug Interactions Gangliosides - pharmacology Guinea Pigs HeLa Cells - drug effects L Cells (Cell Line) - drug effects Macrophages - drug effects Receptors, Drug - drug effects
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Summary:	Adsorption of diphtheria toxin on immobilized cell membrane preparations in the presence of gangliosides was studied. A mixture of gangliosides completely inhibited the adsorption of diphtheria toxin on the cell membranes, both sensitive (HeLa cells, macrophages) and resistant (L-cells) to its action. Neuraminidase-treated gangliosides were less effective. Immobilized gangliosides adsorbed diphtheria toxin effectively in the presence of a protective colloid and detergent triton X-100. The results obtained suggest gangliosides to be diphtheria toxin receptors.
ISSN:	0365-9615