Phospholipase of rat intestine: mode of action

Phospholipase activities of rat intestinal mucosa homogenate have been determined from lysophosphatidylcholines [14C] and phosphatidylcholines [-3H-14C]. In the presence of phosphatidylcholines, at pH 6.5, the homogenate has a phospholipase B activity. At pH 8.5, a phospholipase A2 activity was show...

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Bibliographic Details
Published in:	Biochimie Vol. 59; no. 4; p. 355
Main Authors:	Bonnefis, M J, Rey, G, Thouvenot, J P, Douste-Blazy, L
Format:	Journal Article
Language:	French
Published:	France 1977
Subjects:	Animals Carbon Radioisotopes Hydrogen-Ion Concentration Intestinal Mucosa - enzymology Intestine, Small - enzymology Isotope Labeling Kinetics Lysophosphatidylcholines Phosphatidylcholines Phospholipases - metabolism Rats Structure-Activity Relationship Tritium
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Summary:	Phospholipase activities of rat intestinal mucosa homogenate have been determined from lysophosphatidylcholines [14C] and phosphatidylcholines [-3H-14C]. In the presence of phosphatidylcholines, at pH 6.5, the homogenate has a phospholipase B activity. At pH 8.5, a phospholipase A2 activity was shown. In the presence of lysophospatidylcholines, at pH 6.5, we notice a lysophospholipase A1 activity. A kinetic study of the reactions allows us to separate the activity B into a phospholipase A2 activity and a lysophospholipase A1 activity. Thus, it appears that the total phospholipase activity of rat intestinal mucosa would results from a phospholipase A2 activity and a lysophospholipase A1 activity.
ISSN:	0300-9084