An establishment of murine drug-resistant cell line transfected with human MDR-1 gene.
To investigate the relationship between MDR-1 gene expression and drug resistance in lung cancer cells. The multidrug resistant cell line (3LL-MDR-1) was established by transfecting human MDR-1 gene expressing vector into a murine Lewis lung cancer cell line (3LL) . Drug resistance and the effect of...
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Published in: | Zhongguo fei ai za zhi Vol. 3; no. 1; p. 34 |
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Main Authors: | , , , , |
Format: | Journal Article |
Language: | Chinese |
Published: |
China
Chinese Anti-Cancer Association Chinese Antituberculosis Association
20-02-2000
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Subjects: | |
Online Access: | Get full text |
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Summary: | To investigate the relationship between MDR-1 gene expression and drug resistance in lung cancer cells.
The multidrug resistant cell line (3LL-MDR-1) was established by transfecting human MDR-1 gene expressing vector into a murine Lewis lung cancer cell line (3LL) . Drug resistance and the effect of valapamil (VLP) on the resistance were evaluated by MTT assay. The expression of MDR-1 gene product , P-gp , was measured immunohistochemically. The influx and efflux of Rhodamine 123 was tested with flow cytometry.
The MDR cell line expressing P-gp was established , which exhibited a typical drug resistance to vincristine (VCR) , vindesin (VDS) , etoposide (VP-16) , mitomycin (MMC) , taxol and navelbine (NOR) . Valapamil was able to reverse the resistance of the gene-transfected cells and the reversion was dose-dependent. Flow cytometric analysis showed that the resistant cells had much higher ability to export Rhodamine 123 than the parent cells did. The expression of P-gp protein was visualized immunohistochemi |
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ISSN: | 1009-3419 1999-6187 |
DOI: | 10.3779/cjlc.v3i1.1506 |