Monoclonal antibodies (McAbs) to determinants on human gamma chains: properties of antibodies showing subclass restriction or subclass specificity

Thirteen monoclonal antibodies (McAbs) showing subclass-specific and subclass-restricted reactivity to human IgG have been produced by the hybridoma technique. Their specificity has been defined by passive haemagglutination (HA) and haemagglutination-inhibition (HAI) assays using a large panel pf pu...

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Bibliographic Details
Published in:	Immunology Vol. 47; no. 2; pp. 329 - 336
Main Authors:	Lowe, J, Bird, P, Hardie, D, Jefferis, R, Ling, N R
Format:	Journal Article
Language:	English
Published:	England 01-10-1982
Subjects:	Animals Antibodies, Monoclonal - classification Antibodies, Monoclonal - immunology Antibody Specificity Chromatography, Affinity Epitopes - immunology Hemagglutination Tests Humans Immunodiffusion Immunoglobulin G - classification Immunoglobulin G - immunology Mice Mice, Inbred BALB C
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Summary:	Thirteen monoclonal antibodies (McAbs) showing subclass-specific and subclass-restricted reactivity to human IgG have been produced by the hybridoma technique. Their specificity has been defined by passive haemagglutination (HA) and haemagglutination-inhibition (HAI) assays using a large panel pf purified IgG paraproteins of known subclass. Their use in assaying human IgG subclasses in radial immunodiffusion (RID) and in purifying human subclasses by both positive and negative immunoabsorption chromatography is illustrated.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0019-2805 1365-2567