Nucleotide sequence of a full length cDNA clone encoding for β-tubulin of the sea urchin Paracentrotus lividus

• Published in: Nucleic acids research Vol. 17; no. 14; p. 5851
• Main Authors: DI BERNARDO, M. G, GIANGUZZA, F, CIACCIO, M, PALLA, F, COLOMBO, P, DI BLASI, F, FAIS, M, SPINELLI, G
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 25-07-1989
• Subjects: Amino Acid Sequence; amino acids; Animals; Base Sequence; beta -tubulin; Biological and medical sciences; Blastocyst; cDNA; clones; DNA; DNA - genetics; Echinoidea; Embryo, Nonmammalian; Fundamental and applied biological sciences. Psychology; genes; Genes. Genome; Marine; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; nucleotide sequence; nucleotides; Paracentrotus lividus; proteins; Sea Urchins - embryology; Tubulin - genetics; Paracentrotus lividus; Tubulin; Complementary DNA; Gene; Nucleotide sequence; Echinodermata; Contractile protein; Echinoidea; Invertebrata; Molecular cloning
• ISSN: 0305-1048; 1362-4962

The authors have isolated from a lambda gt 10 cDNA library of P. lividus hatching blastula embryos several clones encoding for beta -tubulin. The DNA sequence of one such clone ( beta 2) containing a 1826 bp insert has been determined. The inferred polypeptide sequence shows that beta contains the coding information for 447 amino acids. Comparison with a human beta -tubulin polypeptide reveals amino acids identity greater than 96%. The amino acid substitutions and the insertions/deletions in the sea urchin beta 2-tubulin, most of which are clustered in the highly acidic C-terminal region of the protein, are indicated with one and three asterisks respectively.