Role of Lipid Rafts in Phagocytic Uptake of Polystyrene Latex Microspheres by Macrophages

• Published in: Anticancer research Vol. 30; no. 8; pp. 3167 - 3176
• Main Authors: NAGAO, Goshi, ISHII, Kazuo, HIROTA, Keiji, MAKINO, Kimiko, TERADA, Hiroshi
• Format: Conference Proceeding Journal Article
• Language: English
• Published: Attiki International Institute of Anticancer Research 01-08-2010
• Subjects: Animals; Biological and medical sciences; Cancer; Cell Line; Endocytosis; Latex - metabolism; Lipid Metabolism; Macrophages - metabolism; Medical sciences; Mice; Microspheres; Phagocytosis; Polystyrenes - metabolism; Tumors; Hevea brasiliensis; Scavenger receptor; Lipid raft; polystyrene latex microspheres; Microsphere; Oligosaccharide; Natural rubber; Lipid rafts; Latex; scavenger receptors; β-Cyclodextrin; Cyclodextrin; Cancerology; methyl-β-cyclodextrin; Dicotyledones; Angiospermae; Euphorbiaceae; Spermatophyta; Phagocytosis; Macrophage
• ISSN: 0250-7005; 1791-7530

Micro- and nanospheres have been used as carrier materials for effective delivery of drugs into macrophages via endocytosis including phagocytosis for the treatment of cancer and infections.
To analyze the role of lipid rafts in the endocytic uptake of microspheres by macrophages, we studied the incorporation of polystyrene latex microspheres (PSL MS) by treating macrophages with reagents that affect the physicochemical and biochemical functions of their lipid rafts, such as the cholesterol depletor methyl-β-cyclodextrin (MβCD) and the inhibitor of scavenger receptors polyinosinic acid (poly I). For this study, we used 3-μm fluorescent polystyrene latex microspheres (F-PSL MS) and J774 murine macrophage.
We found that the endocytosis of J774 cells was remarkably reduced in a concentration-dependent manner by treatment of the cells with MβCD and that endocytosis was suppressed by approximately 50% by treatment with 100 μg/ml poly I. These results suggest that scavenger receptors are associated with phagocytosis of F-PSL MS and that their functions are regulated by lipid rafts.
PSL MS are phagocytosed via a raft-dependent pathway.