Effect of the Gc-derived Macrophage-activating Factor Precursor (preGcMAF) on Phagocytic Activation of Mouse Peritoneal Macrophages

• Published in: Anticancer research Vol. 31; no. 7; pp. 2489 - 2492
• Main Authors: UTO, Yoshihiro, YAMAMOTO, Syota, TAKEUCHI, Ryota, NAKAGAWA, Yoshinori, HIROTA, Keiji, TERADA, Hiroshi, ONIZUKA, Shinya, NAKATA, Eiji, HORI, Hitoshi
• Format: Conference Proceeding Journal Article
• Language: English
• Published: Attiki International Institute of Anticancer Research 01-07-2011
• Subjects: Animals; Arachis hypogaea; Biological and medical sciences; Drug Evaluation, Preclinical; Female; Galactose - metabolism; Glycosylation; Helix pomatia; Macrophage Activation - drug effects; Macrophage-Activating Factors - biosynthesis; Macrophage-Activating Factors - chemistry; Macrophage-Activating Factors - pharmacology; Macrophages, Peritoneal - drug effects; Macrophages, Peritoneal - physiology; Medical sciences; Mice; Mice, Inbred ICR; Molecular Structure; N-Acetylneuraminic Acid - metabolism; Neuraminidase - metabolism; Phagocytosis - drug effects; Protein Precursors - chemistry; Protein Precursors - pharmacology; Protein Processing, Post-Translational; Tumors; Vitamin D-Binding Protein - chemistry; Vitamin D-Binding Protein - metabolism; Gc-Derived macrophage-activating factor precursor; Macrophage activating factor; mouse peritoneal macrophages; subtype of Gc protein; Rodentia; preGcMAF; Precursor; Biological activity; Protein; Vertebrata; Mammalia; Cancerology; Mouse; Animal; phagocytic activity; Gc; Subtype; Phagocytosis; Macrophage; Peritoneum
• ISSN: 0250-7005; 1791-7530

The 1f1f subtype of the Gc protein (Gc(1f1f) protein) was converted into Gc-derived macrophage-activating factor (GcMAF) by enzymatic processing in the presence of β-galactosidase of an activated B-cell and sialidase of a T-cell. We hypothesized that preGc(1f1f)MAF, the only Gc(1f1f) protein lacking galactose, can be converted to GcMAF in vivo because sialic acid is cleaved by residual sialidase. Hence, we investigated the effect of preGc(1f1f)MAF on the phagocytic activation of mouse peritoneal macrophages.
We examined the sugar moiety of preGc(1f1f)MAF with a Western blot using peanut agglutinin (PNA) and Helix pomatia agglutinin (HPA) lectin. We also found that preGc(1f1f)MAF significantly enhanced phagocytic activity in mouse peritoneal macrophages but only in the presence of the mouse peritoneal fluid; the level of phagocytic activity was the same as that observed for GcMAF.
PreGc(1f1f)MAF can be used as an effective macrophage activator in vivo.