Association of interleukin-1 beta (+3954) gene polymorphism and gingival crevicular fluid levels in patients with aggressive and chronic periodontitis

• Published in: Genetic counseling Vol. 24; no. 1; p. 21
• Main Authors: Yücel, O Ozer, Berker, E, Mescil, L, Eratalay, K, Tepe, E, Tezcan, I
• Format: Journal Article
• Language: English
• Published: Switzerland 2013
• Subjects: Adolescent; Adult; Aggressive Periodontitis - genetics; Aggressive Periodontitis - metabolism; Analysis of Variance; Chronic Periodontitis - genetics; Chronic Periodontitis - metabolism; Enzyme-Linked Immunosorbent Assay - methods; Female; Gene Frequency - genetics; Gingival Crevicular Fluid - metabolism; Humans; Interleukin-1beta - genetics; Male; Middle Aged; Periodontal Index; Polymerase Chain Reaction - methods; Polymorphism, Genetic - genetics; Polymorphism, Restriction Fragment Length - genetics; Polymorphism, Single Nucleotide - genetics; Severity of Illness Index; Turkey; Young Adult; Turkey
• ISSN: 1015-8146

The immune mechanisms and genetic variations that regulate genetic expression, production and biological activity of IL-1beta, are thought to play an important role in the pathogenesis of periodontal disease. The aims of the present study were to analyse interleukin (IL)-1beta (+3954) genotype and allele frequency in both chronic and aggressive periodontitis patients, and also to investigate whether this polymorphism is associated with gingival crevicular fluid (GCF) IL-1beta levels, periodontal disease severity and clinical parameters in subjects of Turkish origin.
A total of 147 individuals were enrolled in the study including 56 aggressive periodontitis (AP), 44 chronic periodontitis (CP) patients and 47 healthy controls (C). Single nucleotide polymorphism at IL-1beta (+3954) is analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). GCF samples were analyzed for IL-1beta, using enzyme linked immunosorbent assay (ELISA).
The distribution of genotypes and allele frequencies for IL-1beta (+3954) were similar among the groups, in spite of a trend toward a higher frequency of allele 2 in the patient groups. The genotype distribution and allele frequencies were also not different after stratification of subjects according to the clinical attachment level (CAL < 4 mm and CAL > 4mm). No differences were found between the GCF IL-1beta levels of the different genotypes. Allele 2 was associated with increased bleeding on probing (BOP) sites in chronic periodontitis patients.
The results of this study do not support that genetic polymorphism in the IL-1beta (+3954) could be identified as a susceptibility or severity factor in aggressive periodontitis, in the present population. The association of allele 2 frequency and higher percentage of BOP sites in chronic periodontitis suggest that IL-1beta (+3954) potentially play a significant but not major role in the clinical outcome.