Effects of N-methyl-D-aspartate on quisqualate-stimulated phosphoinositide hydrolysis in slices of kitten striate cortex

• Published in: Brain research Vol. 594; no. 1; p. 146
• Main Authors: Bohner, A P, Dudek, S M, Bear, M F
• Format: Journal Article
• Language: English
• Published: Netherlands 23-10-1992
• Subjects: Animals; Cats; Hydrolysis; In Vitro Techniques; N-Methylaspartate - pharmacology; Phosphatidylinositols - metabolism; Quisqualic Acid - antagonists & inhibitors; Visual Cortex - drug effects; Visual Cortex - metabolism
• ISSN: 0006-8993
• DOI: 10.1016/0006-8993(92)91040-L

Stimulation of phosphoinositide (PI) hydrolysis by excitatory amino acids (EAAs) was studied in coronal slices of kitten visual cortex. Coincubation with N-methyl-D-aspartate (NMDA) markedly reduced the stimulation by quisqualate, however, this inhibition developed with a latency of > 10 min and occurred even when the NMDA exposure preceded, but did not overlap with, incubation in quisqualate. This time-course of NMDA inhibition of EAA-stimulated PI turnover places new constraints on its possible mechanism of inhibition.