Macrophageal infiltration and microvessel density in laryngeal carcinoma: study of 52 cases

• Published in: Acta otorhino-laryngologica italica Vol. 35; no. 5; pp. 321 - 324
• Main Authors: Popov, T M, Dikov, T, Goranova, T E, Stancheva, G, Kaneva, R P, Todorov, S, Stoyanov, O, Rangachev, J, Marinov, T, Popova, D P, Mitev, V I, Konov, D
• Format: Journal Article
• Language: English
• Published: Italy Pacini Editore SpA 01-10-2015
• Subjects: Basic Research in Otorhinolaryngology; Humans; Immunohistochemistry; Laryngeal Neoplasms - blood supply; Microvessels; Neoplasm Recurrence, Local; Neovascularization, Pathologic; Prognosis; CD31; Macrophage count; Laryngeal carcinoma; CD68; Micro-vessel density
• ISSN: 0392-100X; 1827-675X
• DOI: 10.14639/0392-100X-588

Angiogenesis is one of the six originally constituted hallmarks of cancer that has been extensively studied in the last two decades. The aim of our study is to assess the microvessel and macrophageal density in laryngeal carcinoma and its clinicopathological correlations. We immunohistochemically assessed microvessel density (CD34) and macrophage count (CD68) using microarray techniques and then looked for clinicopathological correlations. The mean micro-vessel density in the study group was 14.27 ± 12.92 vessels in a ×200 field with a mean macrophageal infiltration density of 5.19 ± 4.32. Median microvessel density was significantly higher in patients with metastasis than in patients without metastasis. Additionally, linear regression established that macrophageal infiltration density could predict microvessel density in laryngeal carcinoma. We found no association between either factor and recurrence rate or other clinical characteristics. Our study adds additional data to a problem that has been widely studied during the last two decades, even if controversies in this area still remain.