Isolation of Staphylococcus sp. RNA

Isolation of Staphylococcus sp. RNA

Isolation of RNA (ribonucleic acid) is a valuable technique to study gene regulation and functional RNAs. It is important to obtain pure samples of RNA for downstream applications, while avoiding the negative effects of ribonucleases (RNases). Here we describe several methods of extracting RNA, with...

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Bibliographic Details
Published in:Methods in molecular biology (Clifton, N.J.) Vol. 1106; p. 119
Main Author: Eidem, Tess
Format: Journal Article
Language:English
Published: United States 01-01-2014
Subjects:
Reproducibility of Results
RNA, Bacterial - isolation & purification
Staphylococcus - genetics
Online Access:Get more information
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Description
Summary:Isolation of RNA (ribonucleic acid) is a valuable technique to study gene regulation and functional RNAs. It is important to obtain pure samples of RNA for downstream applications, while avoiding the negative effects of ribonucleases (RNases). Here we describe several methods of extracting RNA, with an emphasis on Staphylococcus RNA isolation, and measures that must be taken to ensure high-quality, high-yield RNA.
ISSN:1940-6029
DOI:10.1007/978-1-62703-736-5_10