Sample processing effect on polymerase chain reaction used for identification of Campylobacter jejuni

Model samples of Campylobacter jejuni for polymerase chain reaction (PCR) were prepared by rapid and simple procedures consisting of centrifugation, proteinase K treatment, Chelex 100 treatment, and boiling lyses. A PCR based on specific amplification of the variable sequence of 16S rRNA gene was pe...

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Bibliographic Details
Published in:	Folia microbiologica Vol. 49; no. 6; pp. 693 - 697
Main Authors:	Sabatkova, Z, Pazlarova, J, Demnerova, K
Format:	Journal Article
Language:	English
Published:	United States 2004
Subjects:	Animals Campylobacter - classification Campylobacter - genetics Campylobacter - isolation & purification Campylobacter coli - classification Campylobacter coli - genetics Campylobacter coli - isolation & purification Campylobacter jejuni Campylobacter jejuni - classification Campylobacter jejuni - genetics Campylobacter jejuni - isolation & purification Culture Media Food Microbiology Humans Polymerase Chain Reaction - methods Sodium Chloride
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Summary:	Model samples of Campylobacter jejuni for polymerase chain reaction (PCR) were prepared by rapid and simple procedures consisting of centrifugation, proteinase K treatment, Chelex 100 treatment, and boiling lyses. A PCR based on specific amplification of the variable sequence of 16S rRNA gene was performed using Tth DNA polymerase and the PCR products were visualized by agarose gel electrophoresis. The assay allowed the detection of 10 CFU/mL C. jejuni in the physiological saline and 100 CFU/mL in the basic Park and Sanders broth.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0015-5632
DOI:	10.1007/BF02931551