Salmonella-mediated mucosal cell-mediated immunity

• Published in: Cellular and molecular biology (Noisy-le-Grand, France) Vol. 47; no. 7; p. 1115
• Main Authors: Lillard, Jr, J W, Boyaka, P N, Singh, S, McGhee, J R
• Format: Journal Article
• Language: English
• Published: France 01-11-2001
• Subjects: Animals; Cell Division; Cytokines - metabolism; Female; Immunity, Mucosal - immunology; Immunoglobulin G - immunology; Mice; Mice, Inbred C57BL; Ovalbumin - genetics; Ovalbumin - immunology; Peyer's Patches - immunology; Salmonella typhimurium - genetics; Salmonella typhimurium - immunology; Spleen - immunology; Th1 Cells - cytology; Th1 Cells - immunology; Th1 Cells - metabolism; Th2 Cells - cytology; Th2 Cells - immunology; Th2 Cells - metabolism
• ISSN: 0145-5680

Oral immunization with the recombinant Salmonella typhimurium strain (BRD 847) expressing the C fragment of tetanus toxin (TT) induces brisk Ag-specific mucosal S-IgA and serum Ab responses characterized by strong IgG2a Abs to the encoded antigen. We have constructed an attenuated Salmonella typhimurium (aroA- aroD-) strain that expresses chicken egg albumin (OVA) to further elucidate the role of Salmonella-induced Th1 cell phenotype on mucosal cell-mediated immunity (CMI). Peyer's patches and spleen lymphocytes from mice that received the oral Salmonella-OVA vaccine showed dramatic increases in the percent cell lysis of the H-2b restricted EG7.OVA tumor cell line. These results indicate that a single dose of rSalmonella vaccine antigen vector is required to illicit systemic and mucosal Th1-type responses and CTLs. These results also support the existence of a highly regulated relationship between specific cell-mediated immunity and a branch of the humoral immune system, i.e. mucosal IgA responses.