Sexual difference in properties of 5 alpha-reductase in microsome of rat submandibular glands

The properties of 5 alpha-reductase activity in the submandibular glands of rats were investigated using microsomal fraction in the presence of NADPH, and we found a sexual difference in these properties. The formation of 5 alpha-dihydrotestosterone and 5 alpha-androstane-3 alpha, 17 beta-diol from...

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Bibliographic Details
Published in:	Biochemistry international Vol. 15; no. 4; p. 761
Main Authors:	Koshika, S, Hosaka, M, Tarui, M, Furuyama, M, Nakayama, Y
Format:	Journal Article
Language:	English
Published:	Australia 01-10-1987
Subjects:	Animals Cholestenone 5 alpha-Reductase Female In Vitro Techniques Kinetics Male Microsomes - enzymology NADP - metabolism Oxidoreductases - metabolism Proteins - isolation & purification Rats Rats, Inbred Strains Sex Factors Steroids - isolation & purification Subcellular Fractions - enzymology Submandibular Gland - enzymology Testosterone - pharmacology
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Summary:	The properties of 5 alpha-reductase activity in the submandibular glands of rats were investigated using microsomal fraction in the presence of NADPH, and we found a sexual difference in these properties. The formation of 5 alpha-dihydrotestosterone and 5 alpha-androstane-3 alpha, 17 beta-diol from testosterone demonstrated 5 alpha-reductase in the rat microsomal fraction of this tissue sample. Apparent michaelis constants (Km) of the 5 alpha-reductase activity for testosterone was 1.02 x 10(-6) M for the female tissue. Microsomal fraction of submandibular glands of male rats had two Kms and were determined as 1.12 x 10(-6) M and 1.01 x 10(-5) M. The lower Km of 5 alpha-reductase for male rats was similar to for the females.
ISSN:	0158-5231