Effects of polyene macrolides on the membrane potential of resting and activated human leukocytes

Membrane potential changes in human polymorphonuclear leukocytes have been studied by monitoring the fluorescence signal of a carbocyanine probe in the presence of calcium ionophore A23187, chemotactic tripeptide f-met-leu-phe, and polyene macrolides. Reduction of the signals triggered by the ionoph...

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Bibliographic Details
Published in:	Biochemistry international Vol. 24; no. 2; p. 307
Main Authors:	Jullien, S, Capuozzo, E, Salerno, C, Crifò, C
Format:	Journal Article
Language:	English
Published:	Australia 01-05-1991
Subjects:	Amphotericin B - pharmacology Anti-Bacterial Agents - pharmacology Calcimycin - pharmacology Choline - pharmacology Fluorescence Polarization Humans Luminol - chemistry Membrane Potentials - drug effects N-Formylmethionine Leucyl-Phenylalanine - pharmacology Neutrophils - chemistry Neutrophils - drug effects Neutrophils - physiology Sodium Chloride - pharmacology
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Summary:	Membrane potential changes in human polymorphonuclear leukocytes have been studied by monitoring the fluorescence signal of a carbocyanine probe in the presence of calcium ionophore A23187, chemotactic tripeptide f-met-leu-phe, and polyene macrolides. Reduction of the signals triggered by the ionophore and the tripeptide, inhibition of the respiratory burst, and changes in the Na+ influx into the cell seem to be the dominant effect of the polyenes. Polyene-induced depolarization does not correlate with the inhibition of the respiratory burst.
ISSN:	0158-5231