Use of the SOS-chromotest spot assay as a screening system for detecting genotoxic compounds in crude plant extracts

• Published in: Alternatives to laboratory animals Vol. 30; no. 1; pp. 87 - 92
• Main Authors: ARRIETA BAEZ, Daniel, ZEPEDA VALLEJO, Gerardo, CANO SANCHEZ, Patricia, BRENA VALLE, Matilde, REYES CHILPA, Ricardo, JIMENEZ ESTRADA, Manuel
• Format: Journal Article
• Language: English
• Published: Nottingham Fund for the Replacement of Animals in Medical Experiments 2002
• Subjects: Biological and medical sciences; Chemical Fractionation; Crystallization; Escherichia coli - genetics; General pharmacology; Medical sciences; Methylation; Mutagenicity Tests; Mutagens - analysis; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Plant Extracts - chemistry; Plants, Medicinal - chemistry; Quercetin - analysis; Rutin - analysis; Salmonella typhimurium - drug effects; Salmonella typhimurium - genetics; Senna Plant; Solutions; Antineoplastic agent; Toxicity; Genotoxicity; Extract; Biological activity; Medicinal plant; Leguminosae; Dicotyledones; Angiospermae; Plant origin; SOS chromotest; Spermatophyta; Detection
• ISSN: 0261-1929

An SOS-chromotest spot assay was used to detect genotoxic compounds in crude plant extracts. The method allows simultaneous testing of extracts from different species in either a liquid or a solid crystalline form. Extracts from two species of the genus Senna, native to the state of Morelos, Mexico, were assayed. Four genotoxic compounds were isolated, and were identified as quercetin and rutin from S. wislizeni, and 5,7-di- O-methylrutin and 5,7-di-O-methylquercetin from S. skinneri. The SOS-chromotest spot assay proved to be useful for activity- guided fractionation at the beginning of screening for genotoxic compounds in crude plant extracts.