Validation of an enzyme immunoassay for the determination of total homocysteine in plasma

In recent years, the determination of homocysteine (Hcy) has become increasingly important, since high levels of Hcy in plasma or serum represent an independent risk factor for occlusive vascular diseases. Nowadays, clinical laboratories use several analytical techniques to measure Hcy, of which hig...

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Bibliographic Details
Published in:	Blood coagulation & fibrinolysis Vol. 11; no. 3; p. 235
Main Authors:	Quintana, I, Freeman, D, Galarza, C, Murua, A, Spence, J D, Kordich, L
Format:	Journal Article
Language:	English
Published:	England 01-04-2000
Subjects:	Homocysteine - blood Humans Immunoenzyme Techniques - methods Sensitivity and Specificity
Online Access:	Get more information
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Summary:	In recent years, the determination of homocysteine (Hcy) has become increasingly important, since high levels of Hcy in plasma or serum represent an independent risk factor for occlusive vascular diseases. Nowadays, clinical laboratories use several analytical techniques to measure Hcy, of which high-performance liquid chromatography (HPLC) is the most popular. Recently, assays for Hcy quantification based on enzyme immunoassays (EIA) have become commercially available. Our group carried out the validation of the Axis method and compared results with those obtained by an established HPLC assay. Intra- and inter-assay coefficients of variation were < or = 8.5%. Compared with HPLC, linear regression analysis showed r=0.984, slope=0.952, intercept = 1.24 /mol/l; Bland-Altman procedure, the mean of the difference EIA-HPLC results = 0.5 micromol/l. Our results suggest that Hcy determinations by both methods are equivalent, and that the Axis assay provides reproducible and reliable data.
ISSN:	0957-5235