Alterations in the distribution of fibronectin and laminin in the diabetic human eye

• Published in: Investigative ophthalmology & visual science Vol. 28; no. 3; pp. 515 - 521
• Main Authors: KOHNO, T, SORGENTE, N, GOODNIGHT, R, RYAN, S. J
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Association for Research in Vision and Ophtalmology 01-03-1987
• Subjects: Aged; Aged, 80 and over; Associated diseases and complications; Biological and medical sciences; Diabetes Mellitus - metabolism; Diabetes. Impaired glucose tolerance; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Fibronectins - metabolism; Humans; Laminin - metabolism; Medical sciences; Middle Aged; Retina - metabolism; Retinal Vessels - metabolism; Tissue Distribution; Vitreous Body - metabolism; Endocrinopathy; Human; Eye disease; Laminin; Retinopathy; Diabetes mellitus; Glycoproteins; Indirect immunofluorescence; Fibronectin; Immunological method
• ISSN: 0146-0404; 1552-5783

The distribution of fibronectin and laminin in diabetic human eyes was determined by indirect immunofluorescent techniques. The intense fluorescence suggests increased amounts of fibronectin and laminin in the diabetic internal limiting membrane (ILM). A double laminated pattern of fluorescence for both glycoproteins suggests structural abnormalities of the ILM of the posterior retina. Preretinal and subretinal proliferative tissues fluoresced strongly and diffusely with antifibronectin. This study indicates that in diabetic patients, the ILM, especially in the posterior retina, is biochemically and morphologically abnormal.