Simplified procedure for transient transformation of plant protoplasts using polyethylene glycol treatment

A modification of the polyethylene glycol-mediated transformation procedure which eliminates the manual polyethylene glycol dilution step is presented. A transformation mixture of protoplasts, DNA and polyethylene glycol was plated directly onto agarose blocks after incubation. The procedure was sim...

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Bibliographic Details
Published in:	BioTechniques Vol. 10; no. 3; p. 294
Main Authors:	Lyznik, L A, Peng, J Y, Hodges, T K
Format:	Journal Article
Language:	English
Published:	England 01-03-1991
Subjects:	DNA, Recombinant Glucuronidase - genetics Glucuronidase - metabolism Kinetics Plants - genetics Plasmids Polyethylene Glycols - pharmacology Protoplasts Transformation, Genetic - drug effects Zea mays
Online Access:	Get more information
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Summary:	A modification of the polyethylene glycol-mediated transformation procedure which eliminates the manual polyethylene glycol dilution step is presented. A transformation mixture of protoplasts, DNA and polyethylene glycol was plated directly onto agarose blocks after incubation. The procedure was simple and fast, thereby suitable for screening the gene activity of large numbers of plasmid constructions. It has been tested for both maize and rice protoplasts.
ISSN:	0736-6205