Expression of an active cathepsin B-like protein Sm31 from Schistosoma mansoni in insect cells

Recombinant Schistosoma mansoni cathepsin B was produced in insect cells via the baculovirus expression vector system as a 37.5 kDa precursor molecule and a 31 kDa mature enzyme. Extracts prepared from cells infected with the recombinant virus were able to cleave a synthetic dipeptide substrate spec...

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Bibliographic Details
Published in:	Tropical medicine and parasitology Vol. 43; no. 4; p. 282
Main Authors:	Götz, B, Felleisen, R, Shaw, E, Klinkert, M Q
Format:	Journal Article
Language:	English
Published:	Germany 01-12-1992
Subjects:	Animals Baculoviridae - genetics Cathepsin B - biosynthesis Cathepsin B - genetics Cells, Cultured Genetic Vectors Moths - cytology Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Schistosoma mansoni - enzymology Schistosoma mansoni - genetics
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Summary:	Recombinant Schistosoma mansoni cathepsin B was produced in insect cells via the baculovirus expression vector system as a 37.5 kDa precursor molecule and a 31 kDa mature enzyme. Extracts prepared from cells infected with the recombinant virus were able to cleave a synthetic dipeptide substrate specific for cathepsin B. Proteolytic activity was inhibited by trans-epoxysuccinyl-L-leucyl-amido (4-guanidino) butane (E64) but not by phenylmethylsulphonyl-fluoride (PMSF), pepstatin and 1,10-phenanthroline. Specific inhibition by diazomethylketone derivatives, which bind covalently to the active centre of cysteinyl proteinases was also demonstrated.
ISSN:	0177-2392