Characterization of neuronal regeneration in the abdominal ganglion of Aplysia californica

• Published in: Journal of neurobiology Vol. 35; no. 2; pp. 160 - 172
• Main Authors: Hamilton, John F., Fredman, Steven M.
• Format: Journal Article
• Language: English
• Published: New York John Wiley & Sons, Inc 01-05-1998
• Subjects: Abdomen - innervation; Animals; Aplysia; Aplysia californica; Axons - physiology; Ganglia, Invertebrate; Gills - innervation; Marine; mollusc; Nerve Crush; Nerve Regeneration - physiology; Neural Conduction - physiology; Neuronal Plasticity - physiology; plasticity; regeneration
• ISSN: 0022-3034; 1097-4695
• DOI: 10.1002/(SICI)1097-4695(199805)35:2<160::AID-NEU3>3.0.CO;2-2

The ability of neurons in the abdominal ganglion of Aplysia to regenerate their axons following branchial nerve crush was studied using retrograde staining and intracellular dye injection. The duration of the gill withdrawal reflex (GWR) was measured prior to and following nerve crush. Three days after crushing the nerve, the duration of the gill withdrawal reflex was reduced to 20% of control levels. There was rapid recovery 19 days after crushing the branchial nerve. The GWR duration returned to control levels by postlesion days 25–27. Some of the behavioral recovery can be attributed to axonal regeneration. Regeneration, as evidenced by retrograde staining, was first observed by postlesion day 15. The number of stained neurons in ganglia with crushes increased until postlesion day 33. The number of stained neurons in experimental animals was always less than that of controls (67 ± 9% at postlesion day 56). More axonal regeneration was seen in the hemiganglion ipsilateral to the branchial nerve. Regeneration after 32 days postlesion was 60 ± 5% of controls in the ipsilateral hemiganglion, as opposed to 29 ± 6% in the contralateral hemiganglion. Regeneration of individual neurons was also demonstrated. Identified neuron R2 was shown by intracellular dye injection and electrical stimulation of antidromic action potentials to have an axon in the branchial nerve in all ganglia allowed to regenerate for longer than 32 days. These results indicate that in Aplysia, despite behavioral recovery, complete axonal regeneration does not occur in a large segment of the neurons in the adult central nervous system. © 1998 John Wiley & Sons, Inc. J Neurobiol 35: 160–172, 1998