The effect of short‐term low‐temperature treatments on gene expression in Arabidopsis correlates with changes in intracellular Ca2+ levels

• Published in: Plant, cell and environment Vol. 26; no. 4; pp. 485 - 496
• Main Authors: NORDIN HENRIKSSON, K., TREWAVAS, A. J.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01-04-2003; Blackwell
• Subjects: aequorin; Biological and medical sciences; Ca2+ inhibition; Cell physiology; cold signalling; cooling rate; Fundamental and applied biological sciences. Psychology; LTI78; Molecular and cellular biology; Physical agents; Plant physiology and development; Signal transduction; Vegetative apparatus, growth and morphogenesis. Senescence; Calcium; Ion transport; Ca2+ inhibition; Gene expression; Cytosol; aequorin; Arabidopsis thaliana; cooling rate; Signal transduction; Gene; Cruciferae; Dicotyledones; Angiospermae; Spermatophyta; Experimental plant; Biological accumulation; cold signalling; Low temperature; LT178
• ISSN: 0140-7791; 1365-3040
• DOI: 10.1046/j.1365-3040.2003.00979.x

ABSTRACT
The role of changes in intracellular calcium ion concentration ([Ca2+]i) in low‐temperature signal transduction in plants has lately been supported by several studies. An analysis to determine whether the low‐temperature‐induced increase in cytosolic Ca2+ concentration ([Ca2+]cyt) could be correlated with a downstream response such as gene expression was carried out. The induction of the low‐temperature‐regulated gene LTI78 was used as an end point marker of the signal transduction pathway. It was found that this gene is induced by very brief low‐temperature exposures and that the induction does not depend on a continuous exposure to low temperature. By altering the cooling rate, different patterns of the Ca2+ response were obtained which could be correlated with different patterns of LTI78 induction. Furthermore, reducing the Ca2+ transients by pre‐treatment with the Ca2+ channel blocker La3+ also led to a reduced level of gene induction. The results show that brief exposures to low temperature results in the onset of a signalling pathway that leads to the induction of gene expression. This indicates the involvement of changes in [Ca2+]cyt in low‐temperature signalling leading to LTI78 expression but the presence of multiple signalling pathways is suggested.