Functional genetic variants in apoptosis-associated FAS and FASL genes and risk of bladder cancer in a Turkish population

• Published in: In vivo (Athens) Vol. 28; no. 3; pp. 397 - 402
• Main Authors: Verim, Levent, Timirci-Kahraman, Ozlem, Akbulut, Habib, Akbas, Alpaslan, Ozturk, Tulin, Turan, Saime, Yaylim, Ilhan, Ergen, Arzu, Ozturk, Oguz, Isbir, Turgay
• Format: Journal Article
• Language: English
• Published: Greece 01-05-2014
• Subjects: Aged; Alleles; Case-Control Studies; Epistasis, Genetic; Fas Ligand Protein - genetics; fas Receptor - genetics; Female; Gene Frequency; Genetic Predisposition to Disease; Genetic Variation; Genotype; Humans; Male; Middle Aged; Neoplasm Grading; Neoplasm Staging; Odds Ratio; Polymorphism, Genetic; Risk; Turkey; Urinary Bladder Neoplasms - genetics; Urinary Bladder Neoplasms - pathology; FAS; polymorphism; FAS ligand; bladder cancer; Apoptosis
• ISSN: 1791-7549

The present study aimed to evaluate the role of functional polymorphisms of apoptosis-associated Fatty acid synthase (FAS) and fatty acid synthase ligand (FASL) genes in bladder cancer susceptibility as first presentation in a Turkish population.
Genotypes of 91 patients with bladder cancer and 101 healthy controls were evaluated for the polymorphism of FAS-1377 G/A and FASL-844 T/C genes by polymerase chain reaction and restriction fragment length polymorphism analysis.
The frequency of the FAS-1377 G allele was significantly higher in patients with bladder cancer compared to controls (p<0.001). A significantly increased risk for developing bladder cancer was found for the group bearing a T allele for FASL-844 compared to the homozygous FASL-844 CC genotype (p=0.027). FAS-1377 GG genotype and FASL-844 T allele were found to be independently associated with an increased risk of bladder cancer. Additionally, gene-gene interaction analysis revealed that the frequency of FAS-1377AA with FASL-844TC was significantly lower in patients with bladder cancer in comparison to those of controls (p<0.001). Extensive studies for gene-gene interaction are still needed.
Our study provides new evidence that FAS-1377 G and FASL-844 T alleles may be used as low-penetrant risk factors for bladder cancer development in a Turkish population.