Defluorination as the key trait to gauge the biodegradability of fluorinated pollutants in environmental microbial communities

• Published in: Methods in enzymology Vol. 696; p. 321
• Main Authors: Alexandrino, Diogo A M, Carvalho, Maria F
• Format: Journal Article
• Language: English
• Published: United States 2024
• Subjects: Bacteria - genetics; Bacteria - metabolism; Biodegradation, Environmental; Environmental Microbiology; Environmental Pollutants - metabolism; Fluorine - chemistry; Fluorine - metabolism; Halogenation; Microbiota; Biodegradation; Microbial communities; Fluorinated pollutants; Selective enrichment; Defluorination
• ISSN: 1557-7988
• DOI: 10.1016/bs.mie.2024.02.004

Research on microbial defluorination is largely centred on controlled experiments using axenic or well defined microbial inocula. These approaches serve a relevant purpose in the field, offering fundamental biochemical and mechanistic insights on the intricacies of biological defluorination. However, they fail to account for the effective contribution of environmental microbial communities in the recycling of fluoroorganic pollutants, a highly relevant perspective from an environmental risk assessment standpoint, while also missing an important outlook on how community-wide dynamics can leverage the breakdown of C─F bonds in these recalcitrant compounds. With that in mind, this chapter provides experimental and methodological insights on the study of microbial defluorination in wild environmental communities, using this critical catabolic step as the de facto endpoint to evolve, select and cultivate microorganisms with improved defluorination performances.