Influence of cavity disinfectant and adhesive systems on the bonding procedure in demineralized dentin - a one-year in vitro evaluation

• Published in: The journal of adhesive dentistry Vol. 14; no. 6; p. 575
• Main Authors: Sacramento, Patrícia A, de Castilho, Aline R, Banzi, Efani C, Puppi-Rontani, Regina Maria
• Format: Journal Article
• Language: English
• Published: Germany 01-12-2012
• Subjects: Analysis of Variance; Chlorhexidine - pharmacology; Dental Bonding; Dental Leakage; Dental Stress Analysis; Dentin - pathology; Disinfectants - pharmacology; Drug Interactions; Humans; Resin Cements - pharmacology; Shear Strength; Statistics, Nonparametric; Tooth Demineralization - pathology; Water
• DOI: 10.3290/j.jad.a28730
• ISSN: 1461-5185

To evaluate the influence of a 2% chlorhexidine solution (CHX) on the bond strength and nano leakage of two self-etching adhesive systems on demineralized dentin over a 12-month period.
The middle dentin from sound third molars was exposed and demineralized in vitro. Twelve groups were formed using different adhesive systems (Clearfil Protect Bond [PB], Clearfil SE Bond [SE]) dentin treatment (with or without CHX application), and water-storage times (24 h, 6 and 12 months). Composite resin cylinders were bonded to the prepared dentin, and these specimens underwent microshear bond strength (µSBS) testing and nano leakage evaluation. µSBS data were submitted to a three-way ANOVA and Tukey's test. The failure mode and nano leakage were analyzed descriptively by score.
There was a statistically significant interaction only between the adhesive system and CHX, and adhesive system and water-storage times. SE showed the lowest µSBS just at 24 h water-storage time regardless of CHX. A significant decrease in µSBS values after 6 months of water storage occurred in all of the groups and was maintained until 12 months. Adhesive failure increased with storage time. All groups showed nano leakage at the resin/dentin interfaces and an increased silver deposition was noticed after 6 and 12 months of water storage. The highest percentages of nano leakage were found in CHX groups.
CHX did not interfere with µSBS values for either self-etching adhesive system, but water storage did. Bond strength decreased for both adhesive systems after 6 and 12 months, regardless of CHX application. Nano leakage increased with water-storage time and with CHX application.