An efficient protocol for the generation of monocyte derived dendritic cells using serum-free media for clinical applications in post remission AML patients

Protocols for the generation of dendritic cells (DCs) using serum as a supplementation of culture media leads to reactions due to animal proteins and disease transmissions. Several types of serum-free media (SFM), based on "good manufacture practices" (GMP), have recently been used and see...

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Bibliographic Details
Published in:	Annals of clinical and laboratory science Vol. 44; no. 2; p. 180
Main Authors:	da Silva Simoneti, Gisele, Saad, Sara Teresinha Olalla, Gilli, Simone Cristina Olenscki
Format:	Journal Article
Language:	English
Published:	United States 2014
Subjects:	Adult Cell Culture Techniques - methods Cell Differentiation - drug effects Cell Proliferation - drug effects Cell Survival - drug effects Culture Media, Serum-Free - pharmacology Dendritic Cells - cytology Dendritic Cells - transplantation Dextrans - metabolism Enzyme-Linked Immunosorbent Assay Female Flow Cytometry Fluorescein-5-isothiocyanate - analogs & derivatives Fluorescein-5-isothiocyanate - metabolism Humans Inflammation Mediators - metabolism Interleukin-10 - biosynthesis Interleukin-12 - biosynthesis Leukemia, Myeloid, Acute - therapy Male Monocytes - cytology Phagocytes - cytology Phagocytes - drug effects Phenotype Remission Induction Tissue Donors
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