Processing of proenkephalin-A in bovine chromaffin cells. Identification of natural derived fragments by N-terminal sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry
A large variety of proenkephalin-A-derived peptides (PEAPs) are present in bovine adrenal medulla secretory granules that are cosecreted with catecholamines upon stimulation of chromaffin cells. In the present paper, after reverse phase high performance liquid chromatography of intragranular soluble...
Saved in:
Published in: | The Journal of biological chemistry Vol. 275; no. 49; pp. 38355 - 38362 |
---|---|
Main Authors: | , , , , , , , |
Format: | Journal Article |
Language: | English |
Published: |
United States
08-12-2000
|
Subjects: | |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Abstract | A large variety of proenkephalin-A-derived peptides (PEAPs) are present in bovine adrenal medulla secretory granules that are cosecreted with catecholamines upon stimulation of chromaffin cells. In the present paper, after reverse phase high performance liquid chromatography of intragranular soluble material, PEAPs were immunodetected with antisera raised against specific proenkephalin-A (PEA) sequences (PEA63-70 and PEA224-237) and analyzed by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. Thirty PEAPs were characterized in addition to enkephalins and whole PEA, indicating that preferential proteolytic attacks occurred at both N- and C-terminal regions. A similar approach was used to characterize PEA-derived fragments exocytotically released into the extracellular space that showed five additional minor PEAPs. Among all these naturally generated peptides, enkelytin, the antibacterial bisphos- phorylated C-terminal peptide (PEA209-237), was predominantly generated, as shown by MALDI-TOF mass spectrometry analysis, which constituted an efficient method for its identification. Finally, the data on PEA intragranular and extracellular processing in adrenal medulla are discussed in regard to the known enzymatic processing mechanisms. We note the high conservation of the cleavage points in evolutionarily diverse organisms, highlighting an important biological function for the released PEAPs. |
---|---|
AbstractList | A large variety of proenkephalin-A-derived peptides (PEAPs) are present in bovine adrenal medulla secretory granules that are cosecreted with catecholamines upon stimulation of chromaffin cells. In the present paper, after reverse phase high performance liquid chromatography of intragranular soluble material, PEAPs were immunodetected with antisera raised against specific proenkephalin-A (PEA) sequences (PEA63-70 and PEA224-237) and analyzed by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry. Thirty PEAPs were characterized in addition to enkephalins and whole PEA, indicating that preferential proteolytic attacks occurred at both N- and C-terminal regions. A similar approach was used to characterize PEA-derived fragments exocytotically released into the extracellular space that showed five additional minor PEAPs. Among all these naturally generated peptides, enkelytin, the antibacterial bisphos- phorylated C-terminal peptide (PEA209-237), was predominantly generated, as shown by MALDI-TOF mass spectrometry analysis, which constituted an efficient method for its identification. Finally, the data on PEA intragranular and extracellular processing in adrenal medulla are discussed in regard to the known enzymatic processing mechanisms. We note the high conservation of the cleavage points in evolutionarily diverse organisms, highlighting an important biological function for the released PEAPs. |
Author | Gadroy, P Strub, J M Goumon, Y Welters, I D Metz-Boutigue, M H Lugardon, K Stefano, G B Aunis, D |
Author_xml | – sequence: 1 givenname: Y surname: Goumon fullname: Goumon, Y organization: INSERM Unité 338, Biologie de la Communication Cellulaire, 67084 Strasbourg, France – sequence: 2 givenname: K surname: Lugardon fullname: Lugardon, K – sequence: 3 givenname: P surname: Gadroy fullname: Gadroy, P – sequence: 4 givenname: J M surname: Strub fullname: Strub, J M – sequence: 5 givenname: I D surname: Welters fullname: Welters, I D – sequence: 6 givenname: G B surname: Stefano fullname: Stefano, G B – sequence: 7 givenname: D surname: Aunis fullname: Aunis, D – sequence: 8 givenname: M H surname: Metz-Boutigue fullname: Metz-Boutigue, M H |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/10988298$$D View this record in MEDLINE/PubMed |
BookMark | eNo1UTtv3DAM1pCgebRrx0JTN19lWbbsMQiaNED6GNr5QEvUnVJbckTdIdc_2b9UXZISEAiQ34OkLthJiAEZe1-LVS20-vQwmtVXIXTbainECTsXQtbVINv-jF0QPYgSaqjfsLNaDH0vh_6c_f2RokEiHzY8Or6kiOE3LluYfKiuuA98jHsfkJttijM4VyoGp4lW_M5iyN55A9nHcGQHyLsEE7eY_B4tdwk2cwERHw_8W5UxzT6UPuHjDoM5ekKwfIac_FMFZQrKhTYBYSoiFNPyLF2e__PsUmU_49HKTX6zzYVKxGlBk8t0mNPhLTt1MBG-e82X7NfN55_XX6r777d311f31SKFzpVRKNHq3nV1a2olcKz7YRyEAtBmHBE6pS22puu0tFY1GmQvjO2NNk51nWwu2ccX3XKxsgzl9ezpeBgIGHe01lI1qmubAvzwCtyNM9r1kvwM6bD-_wXNP3ghj_M |
ContentType | Journal Article |
DBID | CGR CUY CVF ECM EIF NPM 7X8 |
DOI | 10.1074/jbc.M007557200 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed MEDLINE - Academic |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) MEDLINE - Academic |
DatabaseTitleList | MEDLINE |
Database_xml | – sequence: 1 dbid: ECM name: MEDLINE url: https://search.ebscohost.com/login.aspx?direct=true&db=cmedm&site=ehost-live sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Anatomy & Physiology Chemistry |
EndPage | 38362 |
ExternalDocumentID | 10988298 |
Genre | Research Support, Non-U.S. Gov't Journal Article |
GroupedDBID | --- -DZ -ET -~X .55 .GJ 0R~ 0SF 186 18M 2WC 34G 39C 3O- 53G 5BI 5GY 5RE 5VS 6TJ 79B 85S AAEDW AAFWJ AALRI AARDX AAXUO ABDNZ ABOCM ABPPZ ABRJW ABTAH ACGFO ACNCT ADBBV ADIYS ADNWM ADVLN AENEX AEXQZ AFFNX AFOSN AFPKN AI. AITUG AKRWK ALMA_UNASSIGNED_HOLDINGS AMRAJ BTFSW C1A CGR CJ0 CS3 CUY CVF DIK DU5 E3Z EBS ECM EIF EJD F20 F5P FA8 FDB FRP GROUPED_DOAJ GX1 H13 HH5 IH2 KQ8 L7B MVM N9A NHB NPM OHT OK1 P-O P0W P2P R.V RHF RHI RNS ROL RPM SJN TBC TN5 TR2 UHB UKR UPT UQL VH1 VQA W8F WH7 WHG WOQ X7M XSW Y6R YQT YSK YWH YYP YZZ ZE2 ZGI ZY4 ~02 ~KM 7X8 |
ID | FETCH-LOGICAL-p207t-c4e2ed78f615c140eb189b904aa7cbbea647de5c6672dd437a280cd8c7cf46623 |
ISSN | 0021-9258 |
IngestDate | Fri Oct 25 03:22:52 EDT 2024 Sat Sep 28 08:30:11 EDT 2024 |
IsDoiOpenAccess | false |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 49 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-p207t-c4e2ed78f615c140eb189b904aa7cbbea647de5c6672dd437a280cd8c7cf46623 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
PMID | 10988298 |
PQID | 72434653 |
PQPubID | 23479 |
PageCount | 8 |
ParticipantIDs | proquest_miscellaneous_72434653 pubmed_primary_10988298 |
PublicationCentury | 2000 |
PublicationDate | 2000-Dec-08 20001208 |
PublicationDateYYYYMMDD | 2000-12-08 |
PublicationDate_xml | – month: 12 year: 2000 text: 2000-Dec-08 day: 08 |
PublicationDecade | 2000 |
PublicationPlace | United States |
PublicationPlace_xml | – name: United States |
PublicationTitle | The Journal of biological chemistry |
PublicationTitleAlternate | J Biol Chem |
PublicationYear | 2000 |
SSID | ssj0000491 |
Score | 1.8261768 |
Snippet | A large variety of proenkephalin-A-derived peptides (PEAPs) are present in bovine adrenal medulla secretory granules that are cosecreted with catecholamines... |
SourceID | proquest pubmed |
SourceType | Aggregation Database Index Database |
StartPage | 38355 |
SubjectTerms | Adrenal Medulla - cytology Adrenal Medulla - metabolism Amino Acid Sequence Animals Cattle Cells, Cultured Chromaffin Cells - metabolism Cricetinae Cytoplasmic Granules - metabolism Enkephalins - chemistry Enkephalins - metabolism Humans Mesocricetus Mice Molecular Sequence Data Peptide Fragments - chemistry Protein Precursors - chemistry Protein Precursors - metabolism Protein Processing, Post-Translational Sequence Alignment Sequence Homology, Amino Acid Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Xenopus laevis |
Title | Processing of proenkephalin-A in bovine chromaffin cells. Identification of natural derived fragments by N-terminal sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry |
URI | https://www.ncbi.nlm.nih.gov/pubmed/10988298 https://search.proquest.com/docview/72434653 |
Volume | 275 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9NAEF6l5QAXBC2P8pwD4hK5JJu11z5GJaWqQjg0lXqL1t7dtiKxozyq9lfyl5jxru0mCAEHLlaylh_yfJ79djzzDWMfsii0yGJ5oJOuxgWKCoMkESpIRCgydIhWR1TvfHImRxfx54EYtFpV87pm7L9aGsfQ1lQ5-w_Wrk-KA_gbbY5btDpu_8ruPvO_SmZeFCb_buZXSLfzoE_RjZRiCKadXS2KmbKW8tDNdLo8bLuaXeuDeGWGh3KqHBpv_AaZqV2oS1cSh6R1FPhEmmnb52NX9Y4zkv2_DZCWE4Z0Gwl62Yd8WSycg6IYsCv_DKi3PV3KTilKgIei4y2rP0lGYbVZr92UsZUE2ulHOYWTqm1dnU5UrGcun6Cm6sP1Jb4NbrCO7X5RuopG1rGm1WJdfqQ69ZHiKibi8kvi-36eEk-4E4Wv_Dx3LVo8oJ1QqnfbuEx3YsGeA-B_N0f8MsEg46IJJs0OvxLbCqUTWt1S8h59mxyfD4eT8eBivMMecHSC5IPPTkcNSxC-m6O_00pQVIpPm2f__dKnpEDjJ-yxf_TQd6B7ylom32P7fURJMbuDj1BmE5efafbYw6PKJPvsR4NJKCxsYRKuc3CYhAaT4DAJm5ikoz0mwWMSakxCegcNJqHBJCAmYQuTUGISGkzCFibpUg6TQJiE-5h8xs6PB-Ojk8A3EgnmvCNXQSYMN1rGFul71hUd5CdxkiYdoZTM0tSoSEhtwiyKJNda9KTicYdEM2RmRYQLhOdsNy9y85KBUjHuzTROg-jSuIp71qguT0Me6iTm5oC9r6w1wadMD0vlplgvJ5KLHokZHrAXzoiTudOToSQQXOcm8as_HvuaPWrg_obt4gth3rKdpV6_K9H1EwlyyFo |
link.rule.ids | 315,782,786,27933,27934 |
linkProvider | Multiple Vendors |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Processing+of+proenkephalin-A+in+bovine+chromaffin+cells.+Identification+of+natural+derived+fragments+by+N-terminal+sequencing+and+matrix-assisted+laser+desorption+ionization-time+of+flight+mass+spectrometry&rft.jtitle=The+Journal+of+biological+chemistry&rft.au=Goumon%2C+Y&rft.au=Lugardon%2C+K&rft.au=Gadroy%2C+P&rft.au=Strub%2C+J+M&rft.date=2000-12-08&rft.issn=0021-9258&rft.volume=275&rft.issue=49&rft.spage=38355&rft.epage=38362&rft_id=info:doi/10.1074%2Fjbc.M007557200&rft.externalDBID=NO_FULL_TEXT |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0021-9258&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0021-9258&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0021-9258&client=summon |