The evaluation of murine pleural lavage fluid cellular composition in experimental hemorrhagic shock with special regard to mast cells morphometry

• Published in: Journal of physiology and pharmacology : an official journal of the Polish Physiological Society Vol. 52; no. 2; pp. 293 - 301
• Main Authors: Kasacka, I, Humeńczyk-Zybała, M, Debek, W, Chyczewski, L, Niczyporuk, M, Myćko, G
• Format: Journal Article
• Language: English
• Published: Poland 01-06-2001
• Subjects: Animals; Bronchoalveolar Lavage Fluid; Cell Count; Female; Mast Cells - pathology; Pleura - pathology; Rats; Rats, Wistar; Shock, Hemorrhagic - pathology; Therapeutic Irrigation
• ISSN: 0867-5910

In the course of a hemorrhagic shock, pathological changes occur, which result in intensifying the insufficiency of various vital organs. It can also lead to the development of the multiorgan dysfunction syndrome (MODS) that is the cause of high posthemorrhagic mortality. As a result of the ischemia in the lung there appear proinflammatory factors that mobilize and activate mast cells, inducing degranulation in them. The aim of the study was the analysis of cellular composition and cytomorphometric evaluation of mast cells present in the lavage fluid from the pleural cavity of rats in a sham operated group and in the group presenting hemorrhagic shock. The results revealed an increase of the total cell count in the lavage fluid from the pleural cavity. In the cytological smears a statistically significant accumulation of inflammatory cells was present, especially neutrophils. The increase in mast cells and eosinophils number was not statistically significant. There was not a change in the morphometric parameters of mast cells except the circularity index. A decline of the circularity index indirectly may suggest the degranulation of mast cells, which reflects an inflammatory process in the lungs.