Analysis of variation in batches of armadillo-derived Mycobacterium leprae by immunoblotting

Analysis of variation in batches of armadillo-derived Mycobacterium leprae by immunoblotting

Several batches of cell-free extracts of armadillo-derived Mycobacterium leprae were analyzed by SDS-PAGE and by immunoblotting with monoclonal antibodies. The presence or absence of protease inhibitors had a profound effect on the protein antigens, particularly the 65-kDa antigen. In the absence of...

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Bibliographic Details
Published in:International journal of leprosy and other mycobacterial diseases Vol. 58; no. 1; p. 73
Main Authors: Ibrahim, M A, Lamb, F I, Colston, M J
Format: Journal Article
Language:English
Published: United States 01-03-1990
Subjects:
Animals
Antibodies, Monoclonal
Antigens, Bacterial - analysis
Armadillos
Electrophoresis, Polyacrylamide Gel
Epitopes - immunology
Immunoblotting
Molecular Weight
Mycobacterium leprae - immunology
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Summary:Several batches of cell-free extracts of armadillo-derived Mycobacterium leprae were analyzed by SDS-PAGE and by immunoblotting with monoclonal antibodies. The presence or absence of protease inhibitors had a profound effect on the protein antigens, particularly the 65-kDa antigen. In the absence of protease inhibitors, there were both quantitative and qualitative differences between the different batches of M. leprae extracts.
ISSN:0148-916X