Production of monoclonal antibodies against the non-specific cross-reacting antigen (NCA)

Hybridomas were prepared by fusion of mouse spleen cells immunized with purified NCA (non-specific cross-reacting antigen) with cells of myeloma SP2/0. After cloning, several clones were obtained which produced antibodies specific for NCA, i.e., not reacting with CEA. All antibodies were IgG1 Kappa....

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Bibliographic Details
Published in:	Oncodevelopmental biology and medicine : the journal of the International Society for Oncodevelopmental Biology and Medicine Vol. 4; no. 3; p. 209
Main Authors:	Chavanel, G, Frenoy, N, Escribano, M J, Burtin, P
Format:	Journal Article
Language:	English
Published:	Netherlands 1983
Subjects:	Animals Antibodies, Monoclonal - biosynthesis Antigens, Neoplasm Carcinoembryonic Antigen - immunology Cell Adhesion Molecules Cross Reactions Glycoproteins - immunology Humans Hybridomas - immunology Immunoglobulin G Immunoglobulin kappa-Chains Mice Mice, Inbred BALB C
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Summary:	Hybridomas were prepared by fusion of mouse spleen cells immunized with purified NCA (non-specific cross-reacting antigen) with cells of myeloma SP2/0. After cloning, several clones were obtained which produced antibodies specific for NCA, i.e., not reacting with CEA. All antibodies were IgG1 Kappa. Their affinity constants were higher at 4 degrees C than at 37 degrees C. They revealed the existence of at least two epitopes on the specific moiety of NCA.
ISSN:	0167-1618