Construction and use of PCR primers from a 70 kDa heat shock protein gene for identification of Candida albicans

Methods for detection of Candida albicans in culture or biological samples were developed by the use of polymerase chain reaction (PCR) with oligonucleotide primers from C. albicans 70 kDa heat shock protein gene (Cahsp70). The PCR amplifies a 335-base pair fragment which is then hybridized with a n...

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Bibliographic Details
Published in:	Molecular and cellular probes Vol. 11; no. 5; pp. 329 - 336
Main Authors:	Arancia, S, Sandini, S, Cassone, A, De Bernardis, F, La Valle, R
Format:	Journal Article
Language:	English
Published:	England 01-10-1997
Subjects:	Amino Acid Sequence Base Sequence Blotting, Southern Candida albicans - genetics Candida albicans - isolation & purification Digoxigenin - metabolism DNA Primers Electrophoresis, Agar Gel Fluorescein Fungal Proteins - genetics HSP70 Heat-Shock Proteins - genetics Humans Molecular Sequence Data Nucleic Acid Hybridization Polymerase Chain Reaction Sequence Analysis Species Specificity
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Summary:	Methods for detection of Candida albicans in culture or biological samples were developed by the use of polymerase chain reaction (PCR) with oligonucleotide primers from C. albicans 70 kDa heat shock protein gene (Cahsp70). The PCR amplifies a 335-base pair fragment which is then hybridized with a non-radioactive probe, leading to the specific identification of C. albicans and its differentiation from all other human pathogenic Candida and/or yeast species. Candida albicans could be rapidly detected in human urine and blood, with a sensitivity of 10 and 50 fungal cells per sample, respectively.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0890-8508
DOI:	10.1006/mcpr.1997.0125