Absence of BoLA class I antigens on bovine spermatozoa

Forty AI bulls were tested for BoLA class I antigens by means of eight specific polyclonal reagents. By means of immobilization and sperm penetration tests these antigens were not detected on sperm cells. Isoimmunization studies with the use of sperm as antigenic stimuli and insemination of frozen s...

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Bibliographic Details
Published in:	Animal genetics Vol. 20; no. 1; p. 65
Main Authors:	Matousek, J, Srsen, V, Simon, M, Horín, P, Louda, F
Format:	Journal Article
Language:	English
Published:	England 1989
Subjects:	Animals Cattle - immunology Histocompatibility Antigens Class I - analysis Lymphocytes - immunology Male Spermatozoa - immunology
Online Access:	Get more information
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Summary:	Forty AI bulls were tested for BoLA class I antigens by means of eight specific polyclonal reagents. By means of immobilization and sperm penetration tests these antigens were not detected on sperm cells. Isoimmunization studies with the use of sperm as antigenic stimuli and insemination of frozen spermatozoa diluted in specific reagents did not prove the presence of BoLA class I antigens on bovine spermatozoa. The cytotoxic tests used in this investigation were not reliable.
ISSN:	0268-9146