Cytoprotective Effect of Gallic Acid against Injuries Promoted by Therapeutic Ionizing Radiation in Preosteoblast Cells
Gallic acid (GA) is a powerful antioxidant extracted from plants of the Brazilian Cerrado. Oxidative stress plays an important role in the occurrence of radiation-induced osteonecrosis in patients treated for head and neck cancer. There is a need to develop research aimed at developing complementary...
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| Published in: | International journal of molecular and cellular medicine Vol. 13; no. 1; pp. 19 - 28 |
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| Main Authors: | , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
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Iran
Babol University of Medical Sciences
2024
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| Abstract | Gallic acid (GA) is a powerful antioxidant extracted from plants of the Brazilian Cerrado. Oxidative stress plays an important role in the occurrence of radiation-induced osteonecrosis in patients treated for head and neck cancer. There is a need to develop research aimed at developing complementary therapies to prevent or reverse bone damage. The aim of the present study was to investigate the effect of GA in preosteoblasts exposed to therapeutic ionizing radiation. MC3T3-E1 preosteoblast cells were treated with 10 µM GA and exposed to 6 Gy ionizing radiation. We performed
assays of cell proliferation, oxidative stress analysis by detection of reactive oxygen species, and alkaline phosphatase assay. GA at lower concentrations was able to significantly increase proliferation and inhibit radiation-induced generation of reactive oxygen species in osteoblast precursor cells, despite ionizing radiation-induced injury. Furthermore, GA significantly increased alkaline phosphatase at a dose of 6 Gy. The findings suggested that GA could attenuate ionizing radiation-induced injuries in osteoblast precursor cells. Moreover,
studies are needed to better investigate the role of GA in osteonecrosis, especially in cancer patients undergoing radiotherapy or taking antiresorptive drugs. |
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| AbstractList | Gallic acid (GA) is a powerful antioxidant extracted from plants of the Brazilian Cerrado. Oxidative stress plays an important role in the occurrence of radiation-induced osteonecrosis in patients treated for head and neck cancer. There is a need to develop research aimed at developing complementary therapies to prevent or reverse bone damage. The aim of the present study was to investigate the effect of GA in preosteoblasts exposed to therapeutic ionizing radiation. MC3T3-E1 preosteoblast cells were treated with 10 µM GA and exposed to 6 Gy ionizing radiation. We performed in vitro assays of cell proliferation, oxidative stress analysis by detection of reactive oxygen species, and alkaline phosphatase assay. GA at lower concentrations was able to significantly increase proliferation and inhibit radiation-induced generation of reactive oxygen species in osteoblast precursor cells, despite ionizing radiation-induced injury. Furthermore, GA significantly increased alkaline phosphatase at a dose of 6 Gy. The findings suggested that GA could attenuate ionizing radiation-induced injuries in osteoblast precursor cells. Moreover, in vivo studies are needed to better investigate the role of GA in osteonecrosis, especially in cancer patients undergoing radiotherapy or taking antiresorptive drugs.Gallic acid (GA) is a powerful antioxidant extracted from plants of the Brazilian Cerrado. Oxidative stress plays an important role in the occurrence of radiation-induced osteonecrosis in patients treated for head and neck cancer. There is a need to develop research aimed at developing complementary therapies to prevent or reverse bone damage. The aim of the present study was to investigate the effect of GA in preosteoblasts exposed to therapeutic ionizing radiation. MC3T3-E1 preosteoblast cells were treated with 10 µM GA and exposed to 6 Gy ionizing radiation. We performed in vitro assays of cell proliferation, oxidative stress analysis by detection of reactive oxygen species, and alkaline phosphatase assay. GA at lower concentrations was able to significantly increase proliferation and inhibit radiation-induced generation of reactive oxygen species in osteoblast precursor cells, despite ionizing radiation-induced injury. Furthermore, GA significantly increased alkaline phosphatase at a dose of 6 Gy. The findings suggested that GA could attenuate ionizing radiation-induced injuries in osteoblast precursor cells. Moreover, in vivo studies are needed to better investigate the role of GA in osteonecrosis, especially in cancer patients undergoing radiotherapy or taking antiresorptive drugs. Gallic acid (GA) is a powerful antioxidant extracted from plants of the Brazilian Cerrado. Oxidative stress plays an important role in the occurrence of radiation-induced osteonecrosis in patients treated for head and neck cancer. There is a need to develop research aimed at developing complementary therapies to prevent or reverse bone damage. The aim of the present study was to investigate the effect of GA in preosteoblasts exposed to therapeutic ionizing radiation. MC3T3-E1 preosteoblast cells were treated with 10 µM GA and exposed to 6 Gy ionizing radiation. We performed assays of cell proliferation, oxidative stress analysis by detection of reactive oxygen species, and alkaline phosphatase assay. GA at lower concentrations was able to significantly increase proliferation and inhibit radiation-induced generation of reactive oxygen species in osteoblast precursor cells, despite ionizing radiation-induced injury. Furthermore, GA significantly increased alkaline phosphatase at a dose of 6 Gy. The findings suggested that GA could attenuate ionizing radiation-induced injuries in osteoblast precursor cells. Moreover, studies are needed to better investigate the role of GA in osteonecrosis, especially in cancer patients undergoing radiotherapy or taking antiresorptive drugs. Gallic acid (GA) is a powerful antioxidant extracted from plants of the Brazilian Cerrado. Oxidative stress plays an important role in the occurrence of radiation-induced osteonecrosis in patients treated for head and neck cancer. There is a need to develop research aimed at developing complementary therapies to prevent or reverse bone damage. The aim of the present study was to investigate the effect of GA in preosteoblasts exposed to therapeutic ionizing radiation. MC3T3-E1 preosteoblast cells were treated with 10 µM GA and exposed to 6 Gy ionizing radiation. We performed in vitro assays of cell proliferation, oxidative stress analysis by detection of reactive oxygen species, and alkaline phosphatase assay. GA at lower concentrations was able to significantly increase proliferation and inhibit radiation-induced generation of reactive oxygen species in osteoblast precursor cells, despite ionizing radiation-induced injury. Furthermore, GA significantly increased alkaline phosphatase at a dose of 6 Gy. The findings suggested that GA could attenuate ionizing radiation-induced injuries in osteoblast precursor cells. Moreover, in vivo studies are needed to better investigate the role of GA in osteonecrosis, especially in cancer patients undergoing radiotherapy or taking antiresorptive drugs. |
| Author | Farias, Lucyana Conceição Batista Gomes, Emisael Stênio Leite, Renata Sousa Sousa Santos, Sérgio Henrique Lima Tabosa, Angeliny Tamiarana Santiago, Laís Sena Guimarães, André Luiz da Rocha, Rogério Gonçalves Rodrigues, Danillo Costa |
| AuthorAffiliation | 1 Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros, Minas Gerais, Brazil 2 Oncological Radiology Center, Hospital Dilson Godinho, Montes Claros, Minas Gerais, Brazil 3 Department of Dentistry, Universidade Estadual de Montes Claros, Minas Gerais, Brazil 4 Institute of Agricultural Sciences, Food Engineering College, Universidade Federal de Minas Gerais, Montes Claros, Minas Gerais, Brazil |
| AuthorAffiliation_xml | – name: 1 Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros, Minas Gerais, Brazil – name: 3 Department of Dentistry, Universidade Estadual de Montes Claros, Minas Gerais, Brazil – name: 2 Oncological Radiology Center, Hospital Dilson Godinho, Montes Claros, Minas Gerais, Brazil – name: 4 Institute of Agricultural Sciences, Food Engineering College, Universidade Federal de Minas Gerais, Montes Claros, Minas Gerais, Brazil |
| Author_xml | – sequence: 1 givenname: Renata Sousa surname: Leite fullname: Leite, Renata Sousa organization: Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros, Minas Gerais, Brazil – sequence: 2 givenname: Rogério Gonçalves surname: da Rocha fullname: da Rocha, Rogério Gonçalves organization: Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros, Minas Gerais, Brazil – sequence: 3 givenname: Angeliny Tamiarana surname: Lima Tabosa fullname: Lima Tabosa, Angeliny Tamiarana organization: Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros, Minas Gerais, Brazil – sequence: 4 givenname: Emisael Stênio surname: Batista Gomes fullname: Batista Gomes, Emisael Stênio organization: Laboratory of Health Science, Postgraduate Program in Health Sciences, Universidade Estadual de Montes Claros, Minas Gerais, Brazil – sequence: 5 givenname: Laís surname: Santiago fullname: Santiago, Laís organization: Oncological Radiology Center, Hospital Dilson Godinho, Montes Claros, Minas Gerais, Brazil – sequence: 6 givenname: Danillo Costa surname: Rodrigues fullname: Rodrigues, Danillo Costa organization: Department of Dentistry, Universidade Estadual de Montes Claros, Minas Gerais, Brazil – sequence: 7 givenname: Sérgio Henrique surname: Sousa Santos fullname: Sousa Santos, Sérgio Henrique organization: Institute of Agricultural Sciences, Food Engineering College, Universidade Federal de Minas Gerais, Montes Claros, Minas Gerais, Brazil – sequence: 8 givenname: André Luiz surname: Sena Guimarães fullname: Sena Guimarães, André Luiz organization: Department of Dentistry, Universidade Estadual de Montes Claros, Minas Gerais, Brazil – sequence: 9 givenname: Lucyana Conceição surname: Farias fullname: Farias, Lucyana Conceição organization: Department of Dentistry, Universidade Estadual de Montes Claros, Minas Gerais, Brazil |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/39156872$$D View this record in MEDLINE/PubMed |
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| Keywords | radiotherapy osteoradion-ecrosis osteonecrosis Gallic acid Antioxidant ionizing radiation |
| Language | English |
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| Title | Cytoprotective Effect of Gallic Acid against Injuries Promoted by Therapeutic Ionizing Radiation in Preosteoblast Cells |
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