RAPD analysis for genetic diversity of two populations of Mystus vittatus (Bloch) of Madhya Pradesh, India

The random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) was applied to analyze the genetic variation of the 2 populations of Mystus vittatus (Bloch) of Madhya Pradesh, India. 10 random 10-mer primers were primarily scored in 3 individuals from each of the 2 locations. Five primers,...

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Bibliographic Details
Published in:	African journal of biotechnology Vol. 8; no. 17; pp. 4032 - 4038
Main Authors:	Garg, R K, Silawat, N, Sairkar, P, Vijay, N, Mehrotra, N N
Format:	Journal Article
Language:	English
Published:	01-09-2009
Subjects:	Mystus vittatus
Online Access:	Get full text
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Summary:	The random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) was applied to analyze the genetic variation of the 2 populations of Mystus vittatus (Bloch) of Madhya Pradesh, India. 10 random 10-mer primers were primarily scored in 3 individuals from each of the 2 locations. Five primers, which gave polymorphism, were selected for polymerase chain reaction (PCR) and used in the final RAPD analysis of 20 individuals collected from 2 sites, namely Bhadwada reservoir (Bhopal) and Mohinisagar reservoir (Gwalior). These primers produced 388 scorable DNA fragments were found, of which 252 (64.98%) were polymorphic, 182 (46.90%) were monomorphic and 14 (3.61%) were unique. RAPD banding patterns, showed variations between and within the populations, while, the morphological variations were negligible.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	1684-5315 1684-5315