Alu DNA Polymorphism of Human Tissue Plasminogen Activator (tPA) Gene in Diabetic Jordanian Patients
Hypercoagulability and hypofibrinolysis are among the symptoms exhibited by diabetic patients. Our study aimed to address the polymorphic nature of Alu DNA fragment in the human tissue plasminogen activator gene within diabetes mellitus (DM) Jordanian patients. Genomic DNA was isolated from 76 DM pa...
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| Published in: | Iranian biomedical journal Vol. 23; no. 6; pp. 423 - 428 |
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| Main Authors: | , , , , , , |
| Format: | Journal Article |
| Language: | English |
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Iran
Pasteur Institute of Iran
01-11-2019
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| Abstract | Hypercoagulability and hypofibrinolysis are among the symptoms exhibited by diabetic patients. Our study aimed to address the polymorphic nature of Alu DNA fragment in the human tissue plasminogen activator gene within diabetes mellitus (DM) Jordanian patients.
Genomic DNA was isolated from 76 DM patients and 60 non-diabetic Jordanian individuals, and the Alu fragment was amplified using PCR.
The results showed that 80% of the non-diabetic Jordanian subjects were homozygotes for the deletion of the Alu fragment (Alu-/-), 16.7% were homozygotes for its insertion (Alu+/+), and 3.3% were heterozygotes (Alu+/-). Besides, 36.8% of the diabetic patients exhibited the Alu-/- or Alu+/- genotype, and 26.3% were Alu+/+. The Alu-/- genotype occurred less frequently in the diabetic individuals.
The high frequency of the Alu-/- genotype constitutes a protective deletion with respect to DM within the normal subjects. |
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| AbstractList | Hypercoagulability and hypofibrinolysis are among the symptoms exhibited by diabetic patients. Our study aimed to address the polymorphic nature of Alu DNA fragment in the human tissue plasminogen activator gene within diabetes mellitus (DM) Jordanian patients.
Genomic DNA was isolated from 76 DM patients and 60 non-diabetic Jordanian individuals, and the Alu fragment was amplified using PCR.
The results showed that 80% of the non-diabetic Jordanian subjects were homozygotes for the deletion of the Alu fragment (Alu-/-), 16.7% were homozygotes for its insertion (Alu+/+), and 3.3% were heterozygotes (Alu+/-). Besides, 36.8% of the diabetic patients exhibited the Alu-/- or Alu+/- genotype, and 26.3% were Alu+/+. The Alu-/- genotype occurred less frequently in the diabetic individuals.
The high frequency of the Alu-/- genotype constitutes a protective deletion with respect to DM within the normal subjects. BackgroundHypercoagulability and hypofibrinolysis are among the symptoms exhibited by diabetic patients. Our study aimed to address the polymorphic nature of Alu DNA fragment in the human tissue plasminogen activator gene within diabetes mellitus (DM) Jordanian patients. MethodsGenomic DNA was isolated from 76 DM patients and 60 non-diabetic Jordanian individuals, and the Alu fragment was amplified using PCR. ResultsThe results showed that 80% of the non-diabetic Jordanian subjects were homozygotes for the deletion of the Alu fragment (Alu-/-), 16.7% were homozygotes for its insertion (Alu+/+), and 3.3% were heterozygotes (Alu+/-). Besides, 36.8% of the diabetic patients exhibited the Alu-/- or Alu+/- genotype, and 26.3% were Alu+/+. The Alu-/- genotype occurred less frequently in the diabetic individuals. ConclusionThe high frequency of the Alu-/- genotype constitutes a protective deletion with respect to DM within the normal subjects. |
| Author | Al-Mazaydeh, Zainab A. Al-Emerieen, Ala'a F. AlHawari, Hussam H. Khadra, Maysa M. Yasin, Salem R. Alassaf, Abeer A. Tahtamouni, Lubna H. |
| AuthorAffiliation | 2 Department of Internal Medicine, Faculty of Medicine, University of Jordan, Amman, Jordan 5 Department of Service Courses, Faculty of Science, Zarqa Private University, Zarqa, Jordan 1 Department of Biology and Biotechnology, Faculty of Science, the Hashemite University, Zarqa, Jordan 4 Department of Obstetrics and Gynecology, Faculty of Medicine, University of Jordan, Amman, Jordan 3 Department of Pediatric, Faculty of Medicine, University of Jordan, Amman, Jordan |
| AuthorAffiliation_xml | – name: 1 Department of Biology and Biotechnology, Faculty of Science, the Hashemite University, Zarqa, Jordan – name: 4 Department of Obstetrics and Gynecology, Faculty of Medicine, University of Jordan, Amman, Jordan – name: 3 Department of Pediatric, Faculty of Medicine, University of Jordan, Amman, Jordan – name: 2 Department of Internal Medicine, Faculty of Medicine, University of Jordan, Amman, Jordan – name: 5 Department of Service Courses, Faculty of Science, Zarqa Private University, Zarqa, Jordan |
| Author_xml | – sequence: 1 givenname: Salem R. surname: Yasin fullname: Yasin, Salem R. organization: Department of Biology and Biotechnology, Faculty of Science, the Hashemite University, Zarqa, Jordan – sequence: 2 givenname: Hussam H. surname: AlHawari fullname: AlHawari, Hussam H. organization: Department of Internal Medicine, Faculty of Medicine, University of Jordan, Amman, Jordan – sequence: 3 givenname: Abeer A. surname: Alassaf fullname: Alassaf, Abeer A. organization: Department of Pediatric, Faculty of Medicine, University of Jordan, Amman, Jordan – sequence: 4 givenname: Maysa M. surname: Khadra fullname: Khadra, Maysa M. organization: Department of Obstetrics and Gynecology, Faculty of Medicine, University of Jordan, Amman, Jordan – sequence: 5 givenname: Zainab A. surname: Al-Mazaydeh fullname: Al-Mazaydeh, Zainab A. organization: Department of Biology and Biotechnology, Faculty of Science, the Hashemite University, Zarqa, Jordan – sequence: 6 givenname: Ala'a F. surname: Al-Emerieen fullname: Al-Emerieen, Ala'a F. organization: Department of Service Courses, Faculty of Science, Zarqa Private University, Zarqa, Jordan – sequence: 7 givenname: Lubna H. surname: Tahtamouni fullname: Tahtamouni, Lubna H. organization: Department of Biology and Biotechnology, Faculty of Science, the Hashemite University, Zarqa, Jordan |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/31104419$$D View this record in MEDLINE/PubMed |
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| Keywords | Diabetes mellitus Alu Polymorphism |
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| Snippet | Hypercoagulability and hypofibrinolysis are among the symptoms exhibited by diabetic patients. Our study aimed to address the polymorphic nature of Alu DNA... BackgroundHypercoagulability and hypofibrinolysis are among the symptoms exhibited by diabetic patients. Our study aimed to address the polymorphic nature of... |
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| SubjectTerms | Alleles Alu Elements - genetics Diabetes Mellitus - genetics Gene Frequency Humans Jordan Polymorphism, Genetic Short Communication Tissue Plasminogen Activator - genetics |
| Title | Alu DNA Polymorphism of Human Tissue Plasminogen Activator (tPA) Gene in Diabetic Jordanian Patients |
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