The Tissue-Specific Splicing of 5"-Terminal Exons of the Oct-1 Gene

• Published in: Molecular biology (New York) Vol. 35; no. 1; p. 28
• Main Authors: Pankratova, E V, Deev, I E, Polanovsky, O L
• Format: Journal Article
• Language: English
• Published: New York Springer Nature B.V 01-01-2001
• Subjects: Proteins
• ISSN: 0026-8933; 1608-3245
• DOI: 10.1023/A:1004894516424

It has been shown that the pre-mRNA of transcription factor Oct-1 undergoes tissue-specific splicing. The Oct-1R isoform is synthesized in mouse lymphoid myeloma cells NS/0 of B series and is not found in other somatic and embryonal cells. The initiation sites of oct-1R mRNA transcription are located at positions -159 and -307 from the AUG codon of the 1L exon. In both cases, no TATA box was found in the region preceding these sites (25-30 bp). A different form of oct-1 mRNA (containing the 1U exon) was also found in NS/0 cells, which is probably synthesized in all cell lines. This isoform differs from oct-1R, in particular, by the structure of 5"-terminal exon, which is the result of alternative splicing.[PUBLICATION ABSTRACT]