Measuring Immune Cell Movement Toward the Soluble Microenvironment of Human Tissues Using a Boyden Chamber-Based Migration Assay

Migration assays are used to measure cell movement toward a variety of chemoattractants in a controlled environment. Here we describe a method for a Boyden chamber-based migration assay using conditioned media generated from the tumor, liver, and visceral adipose tissue of cancer patients.

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Bibliographic Details
Published in:	Methods in molecular biology (Clifton, N.J.) Vol. 2645; p. 231
Main Authors:	Mylod, Eimear, Lysaght, Joanne, Conroy, Melissa J
Format:	Journal Article
Language:	English
Published:	United States 2023
Subjects:	Cell Line, Tumor Cell Migration Assays - methods Cell Movement Chemotactic Factors Chemotaxis Culture Media, Conditioned Humans Boyden chamber Immune cells Chemotaxis Cell migration Chemokines
Online Access:	Get more information
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Description
Summary:	Migration assays are used to measure cell movement toward a variety of chemoattractants in a controlled environment. Here we describe a method for a Boyden chamber-based migration assay using conditioned media generated from the tumor, liver, and visceral adipose tissue of cancer patients.
ISSN:	1940-6029
DOI:	10.1007/978-1-0716-3056-3_14