DNA Isolation Long-Read Genomic Sequencing in Ctenophores

Long-read sequencing has proven the necessity for high-quality genomic assemblies of reference species, including enigmatic ctenophores. Obtaining high-molecular-weight genomic DNA is pivotal to this process and has proven highly problematic for many species. Here, we discuss different methodologies...

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Bibliographic Details
Published in:	Methods in molecular biology (Clifton, N.J.) Vol. 2757; p. 185
Main Authors:	Moraga Amador, David, Kohn, Andrea B, Bobkova, Yelena, Panayotova, Nedka G, Moroz, Leonid L
Format:	Journal Article
Language:	English
Published:	United States 2024
Subjects:	Animals Ctenophora - genetics DNA - genetics DNA - isolation & purification Gene Library Genome - genetics Genomics - methods High-Throughput Nucleotide Sequencing - methods Sequence Analysis, DNA - methods Beroe Ctenophora Basal metazoans Bolinopsis Mnemiopsis PacBio sequencing Long-read sequencing Evolution Genome Pleurobrachia
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Summary:	Long-read sequencing has proven the necessity for high-quality genomic assemblies of reference species, including enigmatic ctenophores. Obtaining high-molecular-weight genomic DNA is pivotal to this process and has proven highly problematic for many species. Here, we discuss different methodologies for gDNA isolation and present a protocol for isolating gDNA for several members of the phylum Ctenophora. Specifically, we describe a Pacific Biosciences library construction method used in conjunction with gDNA isolation methods that have proven successful in obtaining high-quality genomic assemblies in ctenophores.
ISSN:	1940-6029
DOI:	10.1007/978-1-0716-3642-8_7