Monosaccharide Analysis After One-Pot Derivatization Followed by Reverse-Phase Liquid Chromatography Separation and UV/Vis Detection
Derivatization of monosaccharides with 1-phenyl-3-methyl-5-pyrazolone (PMP) introduces two chromophores per sugar molecule. Their separation on a superficially porous C18 reverse-phase column, using common liquid chromatography equipment, results in short analysis times (under 20 min) and high sensi...
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| Published in: | Methods in molecular biology (Clifton, N.J.) Vol. 2788; p. 67 |
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| Format: | Journal Article |
| Language: | English |
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2024
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| Abstract | Derivatization of monosaccharides with 1-phenyl-3-methyl-5-pyrazolone (PMP) introduces two chromophores per sugar molecule. Their separation on a superficially porous C18 reverse-phase column, using common liquid chromatography equipment, results in short analysis times (under 20 min) and high sensitivity (limit of quantitation 1 nmol). This method allows for complex monosaccharide mixtures to be separated and quantified using a reasonably simple and safe derivatization procedure. |
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| AbstractList | Derivatization of monosaccharides with 1-phenyl-3-methyl-5-pyrazolone (PMP) introduces two chromophores per sugar molecule. Their separation on a superficially porous C18 reverse-phase column, using common liquid chromatography equipment, results in short analysis times (under 20 min) and high sensitivity (limit of quantitation 1 nmol). This method allows for complex monosaccharide mixtures to be separated and quantified using a reasonably simple and safe derivatization procedure. |
| Author | Loeppert, Renate Cruz-Rubio, José Manuel Praznik, Werner |
| Author_xml | – sequence: 1 givenname: José Manuel surname: Cruz-Rubio fullname: Cruz-Rubio, José Manuel organization: Plant Functional Genomics Lab, Institute of Molecular Biotechnology, Department of Biotechnology, University of Natural Resources and Life Sciences (BOKU), Vienna, Austria – sequence: 2 givenname: Renate surname: Loeppert fullname: Loeppert, Renate organization: Department of Pharmaceutical Sciences, Division of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Vienna, Austria – sequence: 3 givenname: Werner surname: Praznik fullname: Praznik, Werner organization: Department of Pharmaceutical Sciences, Division of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Vienna, Austria |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/38656509$$D View this record in MEDLINE/PubMed |
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| Keywords | 1-Phenyl-3-methyl-5-pyrazolone Monosaccharide derivatization Superficially porous columns PMP Monosaccharide chromophores Chromatography Carbohydrate analysis |
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| SubjectTerms | Antipyrine - analogs & derivatives Antipyrine - chemistry Chromatography, High Pressure Liquid - methods Chromatography, Reverse-Phase - methods Edaravone - chemistry Monosaccharides - analysis Monosaccharides - chemistry Spectrophotometry, Ultraviolet - methods |
| Title | Monosaccharide Analysis After One-Pot Derivatization Followed by Reverse-Phase Liquid Chromatography Separation and UV/Vis Detection |
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