Changes in concentration of amino acids and other metabolites during hypothermic perfusion of the canine kidney

• Published in: Cryobiology Vol. 23; no. 4; p. 366
• Main Authors: Verkh, L, Freier, D T, Celik, C
• Format: Journal Article
• Language: English
• Published: Netherlands 01-08-1986
• Subjects: Amino Acids - metabolism; Animals; Cold Temperature; Dogs; Female; In Vitro Techniques; Kidney - metabolism; Kinetics; Lactates - metabolism; Male; Perfusion; Pyruvates - metabolism
• ISSN: 0011-2240
• DOI: 10.1016/0011-2240(86)90041-6

Changes in the concentration of amino acids and other metabolites were determined in the perfusate during 24 hr of ex vivo hypothermic perfusion of dog kidneys. There was an increase in concentration of most of the amino acids. Two patterns were identified. One showed an increase in concentrations up to 12 hr, and then a leveling off as exemplified by alanine, serine, and glutamate. The other pattern was one of persistent elevation as exemplified by phenylalanine, threonine, and methionine. Glucose, lactate, pyruvate, sodium, potassium, pH, and pO2 were also measured in the perfusate. The results suggest that a degradation of kidney protein may occur during the first 24 hr of perfusion. The levels of other metabolites measured support the fact that glycolysis is responsible for a considerable portion of the total energy production in the kidney under hypothermia.