Primary culture of adipose-derived stromal cells

This study was aimed to explore the methodology regarding culture, proliferation and purification of adipose-derived stromal cells (ADSCs), and to study their biological characteristics. ADSCs were obtained using type I collagenase digestion method. Cell growth was observed, and cell viability were...

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Bibliographic Details
Published in:	Sheng wu yi xue gong cheng xue za zhi Vol. 31; no. 4; p. 842
Main Authors:	Deng, Chunying, Liu, Bin, Zhang, Jinxia
Format:	Journal Article
Language:	Chinese
Published:	China 01-08-2014
Subjects:	Adipocytes - cytology Adipose Tissue - cytology Cell Differentiation Cell Proliferation Cells, Cultured Neurons - cytology Primary Cell Culture Stem Cells - cytology Stromal Cells - cytology
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Summary:	This study was aimed to explore the methodology regarding culture, proliferation and purification of adipose-derived stromal cells (ADSCs), and to study their biological characteristics. ADSCs were obtained using type I collagenase digestion method. Cell growth was observed, and cell viability were detected under different digestion period by MTT. The ADSCs were then identified and induced. The results showed that adherent cells digested by type I collagenase for 60 min had a strong proliferation capability. After the induction of different inducers these adherent cells could differentiate into nerve cells and fat cells. The best digestion period was proved to be of 60 minutes in the experiment. The results indicate that stem cells with multilineage differentiation ability could be separated from adipose tissue, namely ADSCs.
ISSN:	1001-5515