New polymorphism of FASN gene in chicken

Sequencing, Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) were carried out to detect polymorphism in the last intron of the FASN gene of the Campero broiler line. The analysis of the sequences presents a G to A substitution located at base 459 of the PCR product...

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Bibliographic Details
Published in:	Journal of applied genetics Vol. 45; no. 4; p. 453
Main Authors:	Marrube, Graciela, Rozen, Felisa, Pinto, Gabriel Bernardo, Pacienza, Natalia, Melo, Julián Enrique, Huguet, Miguel Javier, Canet, Zulma, Zandomeni, Ruben, Miquel, Maria Cristina
Format:	Journal Article
Language:	English
Published:	England 2004
Subjects:	Animals Chickens - genetics Fatty Acid Synthases - genetics Female Gene Frequency Genotype Introns - genetics Male Polymerase Chain Reaction Polymorphism, Restriction Fragment Length
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Summary:	Sequencing, Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) were carried out to detect polymorphism in the last intron of the FASN gene of the Campero broiler line. The analysis of the sequences presents a G to A substitution located at base 459 of the PCR product (GenBank accession number J02839, located at base Nr. 1222), resulting in a site recognized by restriction enzymes Hae III and Ava II. Also, the sequence presents a G to A substitution (located at base 603 of the PCR product and Nr. 1366 of the J02839 GenBank accession) resulting in a site recognized by restriction enzyme Pst I. Alleles and genotype frequencies were calculated for endonucleases Hae III, Ava II and Pst I for 44 broilers.
ISSN:	1234-1983