31P MRSI at 7 T enables high‐resolution volumetric mapping of the intracellular magnesium ion content in human lower leg muscles

• Published in: Magnetic resonance in medicine Vol. 88; no. 2; pp. 511 - 523
• Main Authors: Franke, Vanessa L., Breitling, Johannes, Ladd, Mark E., Bachert, Peter, Korzowski, Andreas
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc 01-08-2022
• Subjects: 31P MRSI; 7 T; Adenosine triphosphate; ATP; Chemical equilibrium; Data acquisition; Diabetes mellitus; Homeostasis; human lower leg muscle; Ion concentration; Leg; Legs; Magnesium; Mapping; Muscles; phosphorus; Spatial discrimination; Spatial resolution; Time measurement
• ISSN: 0740-3194; 1522-2594
• DOI: 10.1002/mrm.29231

Purpose
The non‐invasive determination of the free magnesium ion concentration ([Mg2+free]) using 31P MRSI in vivo is of interest in research on various pathologies, e.g. diabetes. The purpose of this study was to demonstrate the potential of 31P MRSI at 7 T to enable volumetric, high‐resolution mapping of [Mg2+free].
Methods
3D 31P MRSI datasets from the lower leg of three healthy volunteers were acquired at B0 = 7 T with a nominal spatial resolution of (8 × 8 × 16) mm3 in 56 min. Volumetric [Mg2+free] maps were calculated based on the quantified local chemical shift difference between the α‐ and β‐resonance of adenosine triphosphate (ATP) considering also local pH values. Mean [Mg2+free] values from three different muscle groups were compared. To demonstrate the potential of reducing the measurement time, the analysis was repeated on the acquired MRSI data retrospectively reconstructed with fewer averages.
Results
The generated [Mg2+free] maps revealed local differences, and mean [Mg2+free] values of (1.08 ± 0.03) mM were found in the tibialis anterior, (0.91 ± 0.04) mM in the soleus and (0.98 ± 0.03) mM in the gastrocnemius medialis. The time‐reduced 28‐min scan resulted in comparable [Mg2+free] maps, and mean values being in agreement with the values from the 56‐min scan.
Conclusion
31P MRSI at 7 T enables volumetric, high‐resolution mapping of free magnesium ion content in human lower leg muscles. The measurement time of the 31P MRSI acquisition can be reduced to 28 min, opening the potential to apply volumetric [Mg2+free] mapping for the investigation of pathologies with altered magnesium homeostasis.